Deck 20: Hematology Automation and Flow Cell Cytometry

Question 1

Which of the following principles utilizes on a change in voltage?

A)Electrical impedance
B)Radiofrequency
C)VCS technology
D)Flow cytometry

Answer 1

A

Question 2

Two-parameter histograms are divided into how many regions to generate statistics on cell subpopulations?

A)2
B)16
C)4
D)3

Answer 2

C

Question 3

The ability to identify cells of the same lineage is accomplished through evaluating what cellular characteristic?

A)Cytoplasmic complexity
B)Antigens on cells using clusters of antibodies known as CDs
C)Antibodies on cells
D)Nuclear chromatin patterns

Answer 3

B

Question 4

Quality control for hematology should be run with which frequency?

A)Every 4 hours of testing
B)Every 8 hours of testing
C)Every 24 hours of testing
D)Every week of testing

Question 5

If QC analysis falls outside the expected values, patient samples can be analyzed until QC is maintained.

Question 6

Graphic representations of cell frequencies versus cell size are seen in which of the following?

A)Scatterplots
B)Dot plots
C)Histograms
D)Contour plots

Question 7

In single-parameter histograms, the x-axis represents which of the following?

A)CD marker
B)Number of cells
C)Fluorescent intensity
D)Cell subpopulations

Question 8

Measuring CD is used to analyze which condition?

A)HIV
B)Anemia
C)MDSs
D)Thalassemia

Question 9

Electrical impedance is used to detect which two cell parameters?

A)Cell size and nuclear complexity
B)Cytoplasm and nuclear complexity
C)Number of cells and cell size
D)Number of cells and nuclear complexity

Question 10

On a histogram, the size, or volume, of cells is plotted on which axis?

A)x-axis
B)y-axis

Question 11

In electrical impedance, the length of the change in voltage represents which of the following?

A)Size of the cell
B)Number of cells
C)Nuclear characteristics
D)Cytoplasmic complexity

Question 12

While a histogram can provide cell number and size, scatterplots can demonstrate what additional cellular characteristics?

A)Nuclear complexity
B)Cellular density
C)Nucleus:cytoplasm ratio
D)Cellular population abnormalities

Question 13

On a histogram, the relative number of cells is plotted on which axis?

A)x-axis
B)y-axis

Question 14

Some instruments provide automated reticulocyte counts.Those that do utilize which stain to identify reticulocytes?

A)Wright's stain
B)Prussian blue stain
C)New methylene blue
D)Crystal violet

Question 15

A three-dimensional graphic representation of cellular characteristics is known as which of the following?

A)Histogram
B)Scatterplot
C)Flagging criteria
D)Opacity

Question 16

Contour plots are used in the reporting of which analysis method?

A)Electrical impedance
B)Flow cytometry
C)Radiofrequency
D)Opacity