Deck 12: Gene Transcription and Rna Modification

Question 1

Which of the following is not an example of a eukaryotic regulatory element?

A) Enhancers
B) Silencers
C) Core promoters
D) Cis-acting elements
E) All of the answers are examples

Answer 1

E

Question 2

The consensus sequences are 5'TTGACA3' and 5'TATAAT3' at -35 and -10 respectively. Which of the following would be most easily recognized by σ factor?

A) 5'TTGAAA3'
B) 5'TAAATT3'
C) 5'TATATA3'
D) 5'TTCAGA3'

Answer 2

A

Question 3

A ribozyme is a catalytic protein that assists in the processing of tRNA.
FALSE

Answer 3

False

Question 4

Which of the following is not part of the basal transcription apparatus?

A) DNA
B) TFIID
C) RNA polymerase II
D) Mediator
E) TFIIB

Question 5

In prokaryotes, a holozyme is formed when which of the following is added to the core enzyme?

A) Start codons
B) b
C) b'
D) σ
E) a

Question 6

Which of the following processes is important for the initiation of translation?

A) Alternative splicing
B) RNA editing
C) 5' capping
D) 3' polyA tailing
E) Trimming

Question 7

What transcripts are made by RNA polymerase I in eukaryotes?

A) snRNA
B) tRNA
C) 5S rRNA
D) 18S rRNA

Question 8

The closed promoter complex consists of all of the following, except .

A) RNA polymerase
B) Transcription factors
C) Double helix DNA
D) Single-stranded DNA

Question 9

Which of the following is the site for the start of transcription?

A) Promoter
B) Terminator
C) Regulation sequences
D) Transcription factors
E) All of the answers may start transcription

Question 10

Which of the following forms of RNA encodes the sequence of amino acids for a functional protein?

A) tRNA
B) snRNA
C) mRNA
D) rRNA
E) scRNA

Question 11

A mutation in which of the following would result in a failure of the RNA polymerase to maintain an open complex?

A) TFIIB
B) TFIID
C) TFIIE
D) TFIIH
E) TFIIF

Question 12

Which of the following provides for a higher level of stability in the mRNA?

A) Alternative splicing
B) RNA editing
C) 5' capping
D) 3' polyA tailing
E) Trimming

Question 13

What is the catalytic subunit of prokaryotic RNA polymerase?

A) a
B) b
C) b'
D) σ
E) More than one of the answers

Question 14

Which of the following allows the RNA polymerase II to interact with enhancers and silencers?

A) Mediator
B) TFIIB
C) TFIID
D) Sigma factors
E) None of the answers are correct

Question 15

A gene that undergoes rho-dependent termination has a mutation in the rut site. How will this influence transcriptional activity?

A) Transcription will go on continuously
B) Transcription will stop immediately because RNA polymerase will sense there is a mutation
C) There will eventually be a termination sequence for transcription to end
D) None of the answers are correct

Question 16

A functional protein would contain the information contained within which of the following regions of DNA?

A) Exons
B) Introns
C) Enhancers
D) Promoters
E) All of the answers are correct

Question 17

In eukaryotic organisms, the processing of the 45S rRNA into 5.8S, 18S, and 28S rRNA occurs where?

A) In the cytoplasm
B) In the nucleolus
C) In the endoplasmic reticulum
D) In the Golgi body
E) Throughout the cell

Question 18

What region in eukaryotic genes contains the majority of regulatory elements?

A) 0 to 50
B) -50 to 0
C) -50 to -100
D) -100 to -150

Question 19

How many basal transcription factors are there?

A) 3
B) 4
C) 5
D) 6

Question 20

Following transcription, the RNA has a complementary sequence of which of the following?

A) Regulatory sequences
B) Termination sequences
C) The coding strand of DNA
D) The template strand of DNA
E) None of the answers are correct

Question 21

What would be the effect on in vitro transcription if you replaced the entire TFIID protein with the single subunit TBP?

A) Transcription could not be terminated
B) The initiation complex could not form
C) RNA polymerase II would never be recruited to carry out elongation
D) There would be no effect

Question 22

Which of the following is true of tRNA processing in

A) RNaseP acts as an endonuclease
B) The recognition sequence of RNaseD is 3'CCA5'
C) RNAseD acts as an endonuclease
D) More than one of the answers
E) coli?

Question 23

The Prinbow box contains a CACAAC consensus sequence located in the -100 region of the promoter.

Question 24

The formation of open complex usually forms in GC rich regions of DNA due to the decreased
number of hydrogen bonds.

Question 25

What snRNP holds the exons in place so that they can be connected after the intron is removed?

A) U1
B) U2
C) U5
D) U6

Question 26

Most eukaryotic genes are collinear.

A) True
B) False
C) It is unknown at this time

Question 27

What structural motif is most commonly used by transcription factors in binding to DNA?

A) Zinc-finger
B) Helix-turn-helix
C) Leucine zipper
D) Homeodomain

Question 28

What is the purpose of an SWI complex?

A) Splice introns
B) Activate gene transcription
C) Form the open complex
D) Act as a mediator to RNA polymerase II

Question 29

What do both the rho-dependent and rho-independent mechanisms of termination have in common?

A) Terminate transcription immediately after the stop codon
B) A sequence rich with A-U base pairs
C) Both require a helicase to separate the DNA-RNA complex
D) Formation of a stem-loop structure

Question 30

cDNA contains introns

A) True
B) False
C) It varies from cDNA to cDNA

Question 31

What basal transcription factor is a helicase?

A) TFIID
B) TFIIH
C) TFIIF
D) TFIIB

Question 32

Sigma factor belongs to a general group of proteins called the transcription factors.

Question 33

What enables the splicing of group I and II introns?

A) Spliceosomes
B) Ribozymes
C) snRNA
D) Poly-A tail

Question 34

Alternative splicing allows an organism to .

A) Carry fewer genes
B) Carry more genes
C) Produce fewer gene products
D) More than one of the answers

Question 35

The mRNA is read in groups of nucleotides called quadrons.

Question 36

What is the purpose of phosphorylating the carboxy terminal domain (CTD)?

A) Convert from initiation to the elongation stage
B) To aid in transcriptional initiation
C) Phosphorylate transcription factors
D) More than one above

Question 37

An R loop experiment was performed on two different mRNA's. The first showed one R loop, while the second showed two R loops separated by a region of double-stranded DNA. What is the composition of these two mRNA's?

A) The first contains no introns and the second two introns
B) The first contains no introns and the second one intron
C) The first contains one intron and the second two introns
D) The first contains two introns and the second one intron

Question 38

What amino acid is the target of histone acetyltransferases?

A) Arginine
B) Lysine
C) Methionine
D) Histidine

Question 39

The unit of transcription at the molecular level is the gene.

Question 40

The RNA polymerase forms a closed complex during the process of elongation during transcription.

Question 41

Basal transcription in eukaryotes involves the action of only the core promoter.

Question 42

The term ribozyme is given to catalytic RNA molecules.

Question 43

The process of RNA editing can alter the base sequence of the mRNA following transcription.