Deck 3: The Microscope

Question 1

To transport a microscope, hold it _ .

A) cradled in both arms
B) close to your body with one arm and cover the top of it with your other arm
C) upright, with one hand on its arm and the other hand supporting its base
D) horizontally, with one hand supporting the objective lenses

Answer 1

C

Question 2

When you want to study a slide under the microscope, you place it on the .

A) head
B) stage
C) arm
D) base

Answer 2

B

Question 3

When observing a slide under a microscope, begin focusing with the -power objective lens in place.

A) midrange
B) highest
C) lowest

Answer 3

C

Question 4

The working distance for a 10× objective lens is .

A) a little bit greater than the working distance for the 100× objective lens
B) much less than the working distance for the 100× objective lens
C) much greater than the working distance for the 100× objective lens
D) a little bit less than the working distance for the 100× objective lens

Question 5

You are observing the letter "e" under a compound microscope. Which of the following indicates how the image appears as you view it through the ocular lens?

A) e
B) a
C) E
D) e

Question 6

The microscope field is the _.

A) area you see through the lenses of the microscope
B) space near the microscope where you sit
C) platform on which the slide rests
D) area in which you place the microscope

Question 7

You begin your observations using the lens and the adjustment knob.

A) lowest-power, coarse
B) midrange-power, coarse
C) highest-power, fine
D) midrange-power, fine

Question 8

The total magnification of an object can be found by _ .

A) multiplying the power of the objective lens by the power of the ocular lens
B) multiplying the power of the objective lens by the number of objective lenses on your microscope
C) dividing the power of the objective lens by the power of the ocular lens
D) adding the power of the objective lens and the power of the ocular lens

Question 9

Microscope specimens have depth, as well as length and width. If you are focused on the middle layer of cells and wish to see the top layer of cells clearly, you should move the _.

A) slide toward or away from you
B) lens further away from the slide
C) slide to the left or right
D) lens closer to the slide

Question 10

Before putting away the microscope in the storage cabinet you must observe all of the following except .

A) wrap the cord around the microscope
B) clean the lenses
C) remove the slide from the stage
D) rotate the highest power objective lens in position

Question 11

When using the higher power objective lenses, you would use this part of the microscope to focus the specimen.

A) fine adjustment knob
B) course adjustment knob
C) iris diaphragm lever

Question 12

This is the formula for calculating the diameter of an unknown microscope field.

A) diameter of field A × total magnification of field A = diameter of field B × total magnification of field B
B) diameter of field A + diameter of field B = total magnification of field A + total magnification of field B
C) diameter of field A × diameter of field B = total magnification of field A × total magnification of field B
D) diameter of field A + total magnification of field A = diameter of field B + total magnification of field B

Question 13

If the diameter of your microscope field is 1 mm at low power (10×) and your ocular lens is 10×, then what is the diameter of your field when you switch to the 50× objective lens?

A) 0.02 mm
B) 2 mm
C) 0.002 mm
D) 0.2 mm

Question 14

The ability to discriminate two close objects as separate is called .

A) parfocal
B) virtual image
C) working distance
D) resolution (or resolving power)

Question 15

You have learned that the objective lens magnifies the object on the slide, producing the _ image, and that the ocular lens magnifies that image, forming the _ image.

A) ocular, objective
B) virtual, ocular
C) objective, real
D) real, virtual

Question 16

As you increase the total magnification of the specimen, the size of the field .

A) decreases
B) remains the same
C) increases

Question 17

The is a substage lens that concentrates light on the specimen.

A) condenser
B) light source
C) iris diaphragm lever
D) objective lens

Question 18

The of the microscope carries three or four objective lenses.

A) nosepiece
B) ocular
C) arm
D) base

Question 19

If your ocular lens is 10×, and you are using your 45× objective lens to view a slide, the total magnification is .

A) 250×
B) 450×
C) 55×
D) 90×

Question 20

As you increase the total magnification of the specimen, the working distance .

A) decreases
B) remains the same
C) increases

Question 21

As total magnification increases, the depth of field _.

A) increases
B) decreases
C) remains the same

Question 22

If a circular object seen in your low-power field (diameter 1 mm) occupies about 1/4 of the diameter of the field, the object's diameter is about _.

A) 2.5 µm
B) 0.25 µm
C) 250 µm
D) 25 µm

Question 23

You are looking at a slide of three crossed threads. Yellow is on the bottom, blue is in the middle, and red is on the top. When you rotate the adjustment knob forward (away from you) on your microscope, the stage rises. You move the adjustment knob to focus on the middle thread. As you rotate the adjustment knob forward, which color thread will come into focus next?

A) red
B) blue
C) yellow

Question 24

You prepare a wet mount of your own cheek cells and place it under the microscope, but find that you cannot see any detail of the cells. What can you do to make details more visible?

A) view at a higher power
B) view at a lower power
C) prepare another slide and stain with methylene blue
D) increase light intensity

Question 25

You are looking at very lightly stained cells under the microscope, and you want to increase the contrast so that you can see them "better." You _ the iris diaphragm to _ the light.

A) open, dim
B) close, increase
C) open, increase
D) close, dim