Question 1

Laser is an acronym for&#10;A)light amplification by stimulated emission of radiation.&#10;B)light augmentation by stimulated emitted radiation.&#10;C)light amplified by stimulated energy radiation.&#10;D)large angle stimulation by emitted radiation.

Accepted Answer

Laser stands for "light amplification by stimulated emission of radiation," which accurately describes the process of producing coherent light through the stimulated emission of photons from excited atoms or molecules.

Question 2

A photon is a&#10;A)basic unit of light.&#10;B)basic unit of all radiation.&#10;C)component of an atom.&#10;D)component of laser light.

Accepted Answer

basic unit of all radiation.

Question 3

&#10;Match the activity with the appropriate phase of testing related to laboratory automation.&#10;Documentation of quality control data&#10;A)Preanalytical&#10;B)Analytical&#10;C)Postanalytical

Accepted Answer

Documentation of quality control data is a postanalytical activity, as it involves handling and recording information after the analysis has been completed to ensure the accuracy and reliability of the test results.

Question 4

Laser light is concentrated.

Accepted Answer

Laser light is concentrated because it is coherent, meaning the light waves are in phase in both time and space, allowing the light to be focused to a tight spot.

Question 5

Lasers sort the energy in atoms and molecules, concentrate it, and release it in powerful waves.

Accepted Answer

Lasers work by stimulating atoms or molecules to emit light at particular wavelengths and amplify that light, typically producing a very narrow beam of radiation. The process involves energizing the atoms or molecules (pumping them) to a higher energy state. When they return to a lower energy state, they release their energy in the form of light. This light is then concentrated and can be emitted as a powerful, coherent beam.

Question 6

The most frequently used fluorochrome(s) is (are)&#10;A)fluorescein isothiocyanate (FITC).&#10;B)phycoerythrin (PE).&#10;C)peridinin chlorophyll protein (PerCP).&#10;D)All of the above.

Accepted Answer

All of the options listed, fluorescein isothiocyanate (FITC), phycoerythrin (PE), and peridinin chlorophyll protein (PerCP), are commonly used fluorochromes in various applications, including flow cytometry and fluorescence microscopy.

Question 7

Blood specimens for flow cytometry analysis should be refrigerated.

Accepted Answer

Blood specimens for flow cytometry analysis should not be refrigerated because cold temperatures can adversely affect the viability of cells and the expression of surface markers, which are critical for accurate analysis.

Question 8

&#10;Match the activity with the appropriate phase of testing related to laboratory automation.&#10;Specimen labeling&#10;A)Preanalytical&#10;B)Analytical&#10;C)Postanalytical

Accepted Answer

Specimen labeling is a preanalytical activity, as it occurs before any analysis or testing is performed on the specimen. This phase includes all steps taken from the time a test is ordered until the specimen is prepared and ready for analysis.

Question 9

&#10;Match the activity with the appropriate phase of testing related to laboratory automation.&#10;Validation of results&#10;A)Preanalytical&#10;B)Analytical&#10;C)Postanalytical

Accepted Answer

The answer of &#10;Match the activity with the appropriate phase...

Question 10

The major application(s) of flow cell technology is (are)&#10;A)identification of cells.&#10;B)cell sorting before further analysis.&#10;C)diagnosis of autoimmune disease.&#10;D)Both A and B.

Accepted Answer

Flow cell technology is primarily used for the identification of cells and cell sorting before further analysis, making both A and B correct applications.

Question 11

Tissue specimens require disaggregation before analysis by flow cytometry.

Accepted Answer

The answer of Tissue specimens require disaggregation before analysis by...

Question 12

Current fluorescent methods can perform up to _______-color analysis.&#10;A)two&#10;B)four&#10;C)eight&#10;D)sixteen

Accepted Answer

The answer of Current fluorescent methods can perform up to...

Question 13

A fluorochrome is a(n)&#10;A)dye.&#10;B)immunologic reagent.&#10;C)nonspecific indicator.&#10;D)Both A and B.

Accepted Answer

The answer of A fluorochrome is a(n)&#10;A)dye.&#10;B)immunologic reagent.&#10;C)nonspecific indicator.&#10;D)Both A...

Question 14

The most common type of laser light used in laboratory instrumentation is neon.

Accepted Answer

The answer of The most common type of laser light...

Question 15

The principle of flow cytometry is based on&#10;A)antigen-antibody reactions.&#10;B)the formation of immune complexes.&#10;C)the reaction of dyes with the cellular component of interest.&#10;D)the reaction of dyes with immune complexes.

Accepted Answer

The answer of The principle of flow cytometry is based...

Question 16

Laser light is multicolored.

Accepted Answer

The answer of Laser light is multicolored....

Question 17

Immunophenotyping includes the detection or identification of&#10;A)CD34+ stem cells.&#10;B)minimal residual disease in patients with leukemia.&#10;C)T and B lymphocytes.&#10;D)All of the above.

Accepted Answer

The answer of Immunophenotyping includes the detection or identification of&#10;A)CD34+...

Question 18

&#10;Match the activity with the appropriate phase of testing related to laboratory automation.&#10;Quality control&#10;A)Preanalytical&#10;B)Analytical&#10;C)Postanalytical

Accepted Answer

The answer of &#10;Match the activity with the appropriate phase...

Deck 13: Flow Cytometry