Question 1

Which of the following spectroscopic methods is used as an inexpensive, reliable and easy to couple as a detector?&#10;A) UV-vis spectrometer&#10;B) Raman spectrometer&#10;C) IR spectrometer&#10;D) None of the above

Accepted Answer

The UV-vis spectrometer is commonly used as a detector in chromatography due to its affordability and ease of use. It is also reliable for detecting compounds with chromophores that absorb in the UV or visible range of the electromagnetic spectrum. Raman spectroscopy and IR spectroscopy are both powerful analytical techniques but are typically more expensive and require more expertise to operate.

Question 2

Which of the following methods measures emission of radiation?&#10;A) UV-vis spectrometer&#10;B) Raman spectrometer&#10;C) IR spectrometer&#10;D) Fluorescence spectroscopy

Accepted Answer

Fluorescence spectroscopy measures the emission of radiation. UV-vis, Raman, and IR spectroscopy all measure absorption of radiation.

Question 3

To quantify drugs in urine, scientists may use:&#10;A) Raman spectroscopy&#10;B) IR spectroscopy&#10;C) UV-Vis spectroscopy&#10;D) SEM

Accepted Answer

UV-Vis spectroscopy is commonly used to quantify drugs in urine because many drugs absorb ultraviolet or visible light and can be detected at low concentrations using this technique. Raman spectroscopy and IR spectroscopy are also useful for drug detection, but they are less commonly used for urine analysis. SEM (Scanning Electron Microscopy) is not a suitable technique for drug quantification in urine as it is not a spectroscopic technique and cannot detect small molecules like drugs.

Question 4

Which of the following methods has the highest sensitivity?&#10;A) UV-vis spectroscopy&#10;B) Raman spectroscopy&#10;C) IR spectroscopy&#10;D) Fluorescence spectroscopy

Accepted Answer

Fluorescence spectroscopy has the highest sensitivity among the given options. This is due to its ability to detect even low concentrations of analytes with high signal-to-noise ratio. The absorption of light by a molecule promotes electrons to higher energy states, and when they return to their ground state, they emit light at a longer wavelength. This process amplifies the weak signal from analytes, making fluorescence spectroscopy highly sensitive.

Question 5

Which of the following is not spectroscopic method?&#10;A) Raman spectroscopy&#10;B) IR spectroscopy&#10;C) UV-Vis spectroscopy&#10;D) SEM

Accepted Answer

SEM (scanning electron microscope) is not a spectroscopic method. It is an imaging technique that uses focused beams of electrons to create visualization of a sample's surface. The other three options, Raman, IR, and UV-Vis spectroscopy, all involve the interaction of light with a sample, and are therefore spectroscopic methods.

Question 6

How does UV-Vis spectroscopy work? Explain how it can be applied qualitatively and quantitatively.

Accepted Answer

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Question 7

What is a use of UV-Vis spectroscopy in the forensic setting?

Accepted Answer

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Question 8

What is a use of fluorescence spectroscopy in the forensic setting?

Accepted Answer

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Question 9

How does infrared spectroscopy work? Explain what types of molecules are infrared active and inactive and why they vary in the radiation they absorb.

Accepted Answer

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Question 10

How does Raman spectroscopy work? Explain what types of molecules are Raman active and inactive and why they vary in the radiation they reflect.

Accepted Answer

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Question 11

Contrast Raman spectroscopy with IR spectroscopy.

Accepted Answer

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Question 12

Which spectroscopic method is the best for identification of drugs? Why?

Accepted Answer

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Deck 4: Light Spectroscopy