Deck 6: Chromatography

Question 1

Which of the following has a gas as its mobile phase?

A) GC
B) HPLC
C) CE
D) TLC

Answer 1

A

Question 2

How are samples differentiated using gas chromatography?

A) retention time
B) fluorescence
C) absorption
D) angle scattering

Answer 2

A

Question 3

Which of the following is a technique that can be used to separate a complex mixture of cannabinoids?

A) GC
B) UV-Vis spectroscopy
C) microscopy
D) color tests

Answer 3

A

Question 4

One advantage to liquid chromatography methods over gas chromatography methods is that ____.

A) the samples are heated
B) samples do not need derivatization
C) mass spec can be readily employed
D) runs are typically shorter

Question 5

Which type of material is run in samples with instruments such as gas chromatography and capillary electrophoresis with the sample to calibrate the run for comparison to other runs?

A) internal standard
B) ladder
C) elimination sample
D) reference sample

Question 6

How does thin layer chromatography (TLC) work? Draw a schematic of it implementation and explain what is compared between an analyte and a standard.

Question 7

Define and explain the difference between normal and reverse phase HPLC.

Question 8

How does gas chromatography (GC) work? Draw a schematic of the instrument and label the key features.

Question 9

How can GC be used to quantitate an unknown sample? What samples are run, what is measured and how is this used to calculate the unknown quantity?

Question 10

What are the main differences between HPLC and UPLC?

Question 11

Which samples elute earliest using reverse phase HPLC?

Question 12

Which ignitable liquids elute earliest in GC?