Deck 1: Genetic Engineering and Biotechnology

Question 1

Restriction enzymes were discovered by

A)Smith and Nathans
B)Alexander Fleming
C)Berg
D)None

Answer 1

Smith and Nathans

Question 2

Bacteria protect themselves from viruses by fragmenting viral DNA with

A)Ligase
B)Endonuclease
C)Exonuclease
D)Gyrase

Answer 2

Endonuclease

Question 3

Klenow fragment is derived from

A)DNA Ligase
B)DNA Pol-I
C)DNA Pol-II
D)Reverse Transcriptase

Answer 3

DNA Pol-I

Question 4

Southern blotting is

A)Attachment of probes to DNA fragments
B)Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
C)Comparison of DNA fragments to two sources
D)Transfer of DNA fragments to electrophoretic gel from cellulose membrane

Question 5

ELISA is

A)Using radiolabelled second antibody
B)Usage of RBCs
C)Using complement-mediated cell lysis
D)Addition of substrate that is converted into a coloured end product

Question 6

The Golden Rice variety is rich in

A)Vitamin C
B)?-carotene and ferritin
C)Biotin
D)Lysine

Question 7

The DNA fragments have sticky ends due to

A)Endonuclease
B)Unpaired bases
C)Calcium ions
D)Free methylation

Question 8

Plasmids are used as cloning vectors for which of the following reasons?

A)Can be multiplied in culture
B)Self-replication in bacterial cells
C)Can be multiplied in laboratories with the help of enzymes
D)Replicate freely outside bacterial cells

Question 9

The vaccines prepared through recombinant DNA technology are

A)Third generation vaccines
B)First-generation vaccines
C)Second-generation vaccines
D)None

Question 10

Which is a genetically modified crop?

A)Bt-cotton
B)Bt-brinjal
C)Golden rice
D)All

Question 11

The first transgenic plant to be produced is

A)Brinjal
B)Tobacco
C)Rice
D)Cotton

Question 12

RNA interference helps in

A)Cell proliferation
B)Micropropagation
C)Cell defence
D)Cell differentiation

Question 13

Which of the following is the quality of improved transgenic basmati rice?

A)Gives high yield but no characteristic aroma
B)Gives high yield and is rich in vitamin A
C)Does not require chemical fertilizers and growth hormones
D)Resistant to insects and diseases

Question 14

The first clinical application of gene therapy over a 4 year old girl was for

A)Adenosine deaminase deficiency
B)Adenosine deficiency
C)Growth deficiency
D)Adenine deficiency

Question 15

Excision and insertion of a gene is called

A)Biotechnology
B)Genetic engineering
C)Cytogenetics
D)Gene therapy

Question 16

The expression of a transgene in the target tissue is identified by a

A)Transgene
B)Promoter
C)Enhancer
D)Reporter

Question 17

---- is used as a vector for cloning into higher organisms

A)Retrovirus
B)Baculovirus
C)Salmonella typhimurium
D)Rhizopus nigricans

Question 18

Which bacterium is used in the production of insulin by genetic engineering?

A)Saccharomyces
B)Rhizobium
C)Escherichia
D)Mycobacterium

Question 19

The Taq polymerase enzyme is obtained from

A)Thermus aquaticus
B)Thiobacillus ferrooxidans
C)Bacillus subtilis
D)Pseudomonas subtilis

Question 20

Which of the following is an endonuclease?

A)DNase I
B)Hind II
C)Protease
D)RNase restriction

Question 21

Which of the following restriction enzymes produce blunt ends?

A)Hind III
B)ho
C)Eco RV
D)Sal I

Question 22

Which of the following is not a component of downstream processing?

A)Expression
B)Preservation
C)Purification
D)Separation

Question 23

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

A)Taq polymerase
B)Polymerase III
C)Ligase
D)Eco RI