Question 1

LIS interfaces are an important component in automation because of its potential in:&#10;A) recognizing errors in sample ID.&#10;B) flagging discrepancies with previous test results.&#10;C) providing a mechanism for the electronic crossmatch.&#10;D) All of the above

Accepted Answer

The laboratory information system interface (LIS)is an essential component that provides for correct sample recognition and comparison with prior records; in this function,LIS meets some of the criteria for the electronic crossmatch.

Question 2

A smooth layer of cells in the bottom of the well is interpreted as a/an ____________ reaction when the solid-phase red cell adherence method is completed for the antiglobulin test:&#10;A) positive&#10;B) negative&#10;C) indeterminate&#10;D) mixed field

Accepted Answer

A smooth monolayer of cells indicates a positive reaction,while a compact button is a negative reaction.

Question 3

What does a negative reaction in the MTS Gel Test look like in the gel card?&#10;A) pellet of cells at the bottom of the column.&#10;B) layer of cells at the top of the column.&#10;C) cells evenly distributed throughout the column.&#10;D) cells distributed only in the bottom half of the column.

Accepted Answer

The cells at the bottom of the column indicate a negative reaction because no agglutination took place with the antibody suspended in the gel.

Question 4

What is an advantage of the MTS gel technology over test tubes when performing panels?&#10;A) shorter centrifugation time.&#10;B) decreased reagent cost.&#10;C) reduced patient sample requirements.&#10;D) the incubation step is optional.

Accepted Answer

The patient sample requirement is less for the gel test compared to tube testing.Centrifugation time is longer with gel tests,incubation time is about the same,and the reagent cost associated with gel testing is higher.

Question 5

Random access is an important feature in selecting an automated instrument for testing in the transfusion service.What is random access?&#10;A) Ability of multiple users to sign into the system&#10;B) The recognition of various types of barcodes&#10;C) The need for minimal maintenance procedures&#10;D) The ability to accommodate STAT testing

Accepted Answer

Random access provides the ability to stop the instrument to accommodate STAT tests without disrupting prior sample testing.

Question 6

The determination of the ABO and D type by the NEO and ECHO uses the same solid-phase red cell adherence technique that is used for the antibody screen.

Accepted Answer

The screen uses the solid phase red cell adherence technique,while ABO and D typing uses the hemagglutination technique similar to tube typing.

Question 7

The TANGO,PROVUE,and NEO all use one of the following reagents or components in the indirect antiglobulin test.&#10;A) Washing mechanism&#10;B) Centrifuge&#10;C) Indicator cells&#10;D) Antihuman globulin

Accepted Answer

All systems require a centrifugation step.The gel technology does not require washing or the addition of AHG reagent.Indicator cells (red cells coated with anti-IgG)are used in the NEO and TANGO instruments to detect IgG binding.

Question 8

Which of the following instruments performs assays using gel technology?&#10;A) ECHO&#10;B) PROVUE&#10;C) NEO&#10;D) TANGO

Accepted Answer

The Ortho PROVUE automated platform performs the MTS Gel Test.

Question 9

Which of the following items is required before implementing new automation technology into the transfusion service setting?&#10;A) Validation&#10;B) Training&#10;C) Written procedures&#10;D) All of the above

Accepted Answer

Before implementing a new instrument validation,training and written SOPs must be in place.

Question 10

The PROVUE,TANGO,and NEO have the ability to perform all of the following tests except:&#10;A) ABO/D typing.&#10;B) antibody ID panels.&#10;C) direct antiglobulin test.&#10;D) acid elution.

Accepted Answer

All instruments have the ability to perform ABO/D typing,antibody panels,and the DAT.

Deck 10: Blood Bank Automation for Transfusion Services