Deck 16: Viral, Chlamydial, and Rickettsial Specimens

Question 1

While all of the following are important collection criteria to ensure the recovery of intracellular organisms, which is probably the most important?

A) Material used to collect the specimen
B) Presence of epithelial cells in the specimen
C) Appropriate site of specimen collection
D) Time of collection of the specimen

Answer 1

Presence of epithelial cells in the specimen

Question 2

Which of the following viruses displays seasonality?

A) Influenza
B) Respiratory syncytial
C) Rotavirus
D) All of the above
E) A and B

Answer 2

All of the above

Question 3

Which of the following is not a function of viral transport medium?

A) Provide moisture
B) Preserve the viruses present
C) Inactivation of viruses
D) Control the growth of bacteria and fungi

Answer 3

Inactivation of viruses

Question 4

Sucrose present in 2-SP transport medium is important because it

A) provides nutrients for the viruses present.
B) reduces the negative effect of freezing on enveloped viruses.
C) aids in control of microbial and fungal contamination.
D) helps maintain the pH of the medium.

Question 5

Which of the following specimens should not be refrigerated prior to viral culture?

A) Bone marrow
B) Tissue
C) Body fluid
D) Swab of lesion

Question 6

What portion of a blood sample should be used for testing for the presence of viruses?

A) White blood cells
B) Red blood cells
C) Serum
D) Platelets

Question 7

Primary cell lines, such as primary monkey kidney, can be subcultured

A) indefinitely.
B) 20 to 50 times.
C) 10 to 20 times.
D) 1 or 2 times.

Question 8

Which of the following cells lines usually originate from cancer cells?

A) Continuous
B) Diploid
C) Primary
D) Monolayer

Question 9

Which cell line is the only one that can grow cytomegalovirus?

A) Monolayer
B) Primary
C) Diploid
D) Continuous

Question 10

Cell culture maintenance medium is used to

A) promote rapid cell growth.
B) encourage viral proliferation.
C) prevent contamination with Mycoplasma spp.
D) sustain cells in a steady metabolic state.

Question 11

The functions of the cell roller drum include all of the following except

A) ensuring constant exchange of media.
B) promoting rapid cell growth.
C) improving cell metabolism.
D) improving viral infection of the monolayer.

Question 12

Using the image provided, determine the interpretation of the results of the cell culture.
Source: Sreedharan Athmanathan, Sesha Reddy Bandlapally, and Gullapalli N. Rao.

A) Uninoculated monolayer of cells
B) Positive ELVIS test
C) Cytopathic effect
D) Plaque formation

Question 13

Guinea pig red bloods cells hemadsorb onto a monolayer of cells infected with

A) influenza virus.
B) parainfluenza virus.
C) herpes simplex virus.
D) a and b.
E) b and c.

Question 14

Shell vials are preferred over traditional tube cell culture for the isolation of cytomegalovirus because

A) they are easier to process and read.
B) the detection time is much faster.
C) the cells remain viable longer.
D) CPE produced is diagnostic.

Question 15

The enzyme-linked virus inducible system (ELVIS) identifies herpes simplex virus based on the presence of blue cells in a monolayer. This is an example of

A) cryopreserved cells.
B) co-cultured cells.
C) transgenic cells.
D) shell vial cells.

Question 16

The most common assay used to detect the presence of viruses in clinical material is

A) electron microscopy.
B) molecular techniques.
C) cytohistopathology.
D) immunodiagnosis.

Question 17

Specimen collection for Chlamydia trachomatis includes

A) purulent discharge of urethra.
B) mucus of endocervix.
C) conjunctival scrapings.
D) all of the above.
E) a and b.

Question 18

Recovery of Rickettsia spp. requires processing

A) buffy coat.
B) tissue.
C) swab of petechial rash.
D) a and b.
E) b and c.

Question 19

A McCoy cell line treated with cycloheximide is used for the detection of Chlamydia spp. because the presence of the antibiotic

A) inhibits cell division for better replication of the organism.
B) prevents microbial and fungal contamination.
C) prevents Mycoplasma spp. Contamination.
D) provides more distinct cytopathic effect in the cells.

Question 20

The gold standard, used by most laboratories for detection of Rickettsia spp., is

A) cell line culture.
B) indirect immunofluorescence.
C) latex agglutination.
D) immunoperoxidase.