Question 1

Which of the following is a vector created by the combination of viral DNA fragments and fragments from a plasmid?&#10;A)Cosmid vector&#10;B)Plasmid vector&#10;C)Combined vector&#10;D)Lambda phage vector

Accepted Answer

A cosmid vector is a type of hybrid vector that has the features of a plasmid and a bacteriophage. It contains a plasmid backbone with a modified lambda phage cos site that allows for the insertion of large fragments of DNA, such as viral DNA fragments, into the vector. Therefore, a cosmid vector is the best choice in this scenario as it allows for the combination of viral DNA fragments and fragments from a plasmid.

Question 2

A portion of DNA,such as a gene,that is synthesized from an RNA template is&#10;A)recombinant DNA.&#10;B)probe DNA.&#10;C)complementary DNA.&#10;D)reverse transcriptase.

Accepted Answer

When DNA is synthesized from an RNA template, it is called complementary DNA (cDNA). This process is catalyzed by the enzyme reverse transcriptase, which is commonly used in molecular biology to generate cDNA from messenger RNA (mRNA) templates. Recombinant DNA refers to DNA that has been artificially combined from different sources, while probe DNA refers to a labeled fragment of DNA that is used to detect the presence of a specific sequence in a sample.

Question 3

Enzymes that cut DNA straight across both of the double helix strands produce __________ ends.

Accepted Answer

blunt

Question 4

Reverse transcriptase is a(n)&#10;A)RNA polymerase from the nucleus.&#10;B)reactive RNA probe.&#10;C)synthetic RNA polymerase.&#10;D)DNA polymerase that uses RNA as a template.

Accepted Answer

Reverse transcriptase is a DNA polymerase that synthesizes a complementary DNA (cDNA) strand using an RNA template. It is commonly used in molecular biology for the conversion of RNA to cDNA for downstream applications such as PCR and gene expression analysis.

Question 5

The recombinant protein that is used to help dissolve blood clots such as those that occur in heart attacks and stroke is&#10;A)interferon.&#10;B)factor VIII.&#10;C)antisense molecule.&#10;D)tissue plasminogen activator.

Accepted Answer

Tissue plasminogen activator (tPA) is a recombinant protein that is used to break down blood clots in conditions such as heart attacks and strokes. It works by converting plasminogen to plasmin, which is responsible for degrading the fibrin meshwork that makes up blood clots. Interferon and antisense molecules are not used for this purpose, and factor VIII is used in the treatment of clotting disorders such as hemophilia A.

Question 6

All of the following are steps in the polymerase chain reaction except&#10;A)extension.&#10;B)translation.&#10;C)priming.&#10;D)denaturation.

Accepted Answer

Translation is a step in protein synthesis, not PCR. The steps in PCR are denaturation, annealing of primers, extension of new DNA strands, and repeat cycling of these steps.

Question 7

Recombinant DNA technology is also referred to as __________.

Accepted Answer

The answer of Recombinant DNA technology is also referred to...

Question 8

Which of the following tools of genetic engineering is used to make multiple copies of DNA?&#10;A)PCR&#10;B)Gel electrophoresis&#10;C)Reverse transcription&#10;D)Recombinant DNA

Accepted Answer

PCR (polymerase chain reaction) is a tool used to make multiple copies of a specific segment of DNA. This method involves repeatedly heating and cooling the DNA to separate and replicate the strands, thus amplifying the amount of DNA available for further analysis or manipulation.

Question 9

Which of the following is a true statement about recombinant DNA technology?&#10;A)It is a single technique for genetic manipulation.&#10;B)It involves the modification of an organism's genome.&#10;C)It will replace biotechnology in the future.&#10;D)It manipulates only the genotype.

Accepted Answer

Recombinant DNA technology involves the modification of an organism's genome by inserting or deleting specific genes, thus changing the phenotype of the organism. It is not a single technique, but a collection of techniques that are continuously being developed and refined. It is unlikely to replace biotechnology as it is a fundamental tool in biotechnology. And it manipulates both the genotype and phenotype of an organism.

Question 10

The term endonuclease refers to&#10;A)gene libraries.&#10;B)restriction enzymes.&#10;C)plasmids.&#10;D)cDNA.

Accepted Answer

Endonucleases are also known as restriction enzymes. They are enzymes that cut DNA at specific recognition sequences. They are commonly used in genetic engineering and molecular biology to create recombinant DNA molecules. Gene libraries, plasmids, and cDNA are all related to DNA research, but they are not directly related to endonucleases.

Question 11

Which of the following is NOT an advantage of phage therapy?&#10;A)Specificity of phages to target bacteria&#10;B)Specificity of phages to target viruses&#10;C)Replication of phages at the site of infection&#10;D)No or little side effects

Accepted Answer

The answer of Which of the following is NOT an...

Question 12

The genetic recombination process that occurs when bacteria acquire fragments of DNA from the local environment and then express the encoded proteins is called&#10;A)transcription.&#10;B)transformation.&#10;C)transduction.&#10;D)ligation.

Accepted Answer

The answer of The genetic recombination process that occurs when...

Question 13

Enzymes that can cleave DNA at a particular site are __________.

Accepted Answer

The answer of Enzymes that can cleave DNA at a...

Question 14

A genetic recombination process that uses a cytoplasmic bridge between the donor and the recipient is called&#10;A)transduction.&#10;B)ligation.&#10;C)conjugation.&#10;D)extension.

Accepted Answer

The answer of A genetic recombination process that uses a...

Question 15

The term &#34;biotechnology&#34; was created in 1919 by&#10;A)Robert Koch.&#10;B)Karl Ereky.&#10;C)Robert Bud.&#10;D)Louis Pasteur.

Accepted Answer

The answer of The term &#34;biotechnology&#34; was created in 1919...

Question 16

Small loops of DNA found in the cytoplasm of bacteria are&#10;A)cDNA.&#10;B)mRNA.&#10;C)plasmids.&#10;D)clones.

Accepted Answer

The answer of Small loops of DNA found in the...

Question 17

All of the following codons can code for arginine except&#10;A)CGU.&#10;B)AGA.&#10;C)GGC.&#10;D)AGG.

Accepted Answer

The answer of All of the following codons can code...

Question 18

The synthetic interferon that is used in the treatment of multiple sclerosis is&#10;A)interferon alfa-2b.&#10;B)interferon beta-1a.&#10;C)interferon alfa-2a.&#10;D)interferon gamma-1b.

Accepted Answer

The answer of The synthetic interferon that is used in...

Question 19

In PCR,which of the following temperature ranges is used in the priming process?&#10;A)90-95 &#176;C&#10;B)50-70 &#176;C&#10;C)60-80 &#176;C&#10;D)35-65 &#176;C

Accepted Answer

The answer of In PCR,which of the following temperature ranges...

Question 20

Which of the following scientists was instrumental in the discovery of plasmids?&#10;A)Robert Koch&#10;B)Louis Pasteur&#10;C)Stanley Cohen&#10;D)Willard Johannsen

Accepted Answer

The answer of Which of the following scientists was instrumental...

Question 21

The process of copying a plate to identify a specific colony is called __________.

Accepted Answer

The answer of The process of copying a plate to...

Question 22