Deck 8: Microbiological Laboratory Techniques

Question 1

The destruction of all microorganisms and their endospores is referred to as

A)disinfection.
B)degermination.
C)sanitization.
D)sterilization.

Answer 1

D

Question 2

Which of the following media is often used to grow Mycobacterium tuberculosis?

A)Blood agar
B)MacConkey agar
C)Heart infusion broth
D)Middlebrook's medium

Answer 2

D

Question 3

An inanimate object or substance capable of transporting pathogens from one medium or individual to another is referred to as a

A)vector.
B)fomite.
C)transporter.
D)transposon.

Answer 3

B

Question 4

Which of the following is considered to be a macronutrient for microorganisms?

A)Folic acid
B)Nitrogen
C)Copper
D)Riboflavin

Question 5

In an agar slant,bacterial colonies that appear separate or semiconfluent are referred to as

A)filiform.
B)echinulate.
C)beaded.
D)effuse.

Question 6

A saucer-shaped liquefaction in a gelatin stab is referred to as

A)napiform.
B)saccate.
C)crateriform.
D)stratiform.

Question 7

The process by which the numbers of microbes on the human skin are reduced by scrubbing is called

A)degermination.
B)sanitization.
C)disinfection.
D)sterilization.

Question 8

Which of the following is a biological test organism for validating dry or moist heat sterilization?

A)Bacillus subtilis var.niger
B)Escherichia coli
C)Staphylococcus aureus
D)Staphylococcus epidermidis

Question 9

Which of the following genera of bacteria need very specialized media for culturing?

A)Spiroplasma
B)Enterococcus
C)Staphylococcus
D)Pseudomonas

Question 10

Chemically,agar is a

A)protein.
B)lipid.
C)carbohydrate.
D)nucleic acid.

Question 11

Which of the following stains is generally used for the diagnosis of tuberculosis?

A)Acid-fast stain
B)Gram stain
C)Negative stain
D)Simple stain

Question 12

Which of the following microorganisms can be cultured only in the footpads of mice or a species of armadillo?

A)Mycobacterium tuberculosis
B)Mycobacterium leprae
C)Neisseria gonorrhoeae
D)Mycoplasma

Question 13

Which of the following media are defined and have an exact chemical composition?

A)Complex media
B)Synthetic media
C)Enriched media
D)Special media

Question 14

Which of the following stains is the most widely used differential stain in hematology laboratories?

A)Carbolfuchsin stain
B)Ziehl-Neelson stain
C)Kinyoun stain
D)Wright's stain

Question 15

Which of the following is a general-purpose medium?

A)Tryptic soy agar
B)Blood agar
C)Mannitol salt agar
D)MacConkey agar

Question 16

The stain used to identify bacteria with a large amount of peptidoglycan in their cell walls is the

A)acid-fast stain.
B)methylene blue stain.
C)Gram stain.
D)negative stain.

Question 17

Which of the following media is used to isolate Neisseria gonorrhoeae?

A)Phenylethyl alcohol agar
B)Thayer-Martin agar
C)MacConkey agar
D)Mannitol salt agar

Question 18

Nigrosin is a stain used in

A)simple staining.
B)gram staining.
C)negative staining.
D)acid-fast staining.

Question 19

In correct sequence,the five "I's" in microbiology stand for

A)Inoculation, Inspection, Identification, Incubation, and Isolation.
B)Isolation, Inoculation, Inspection, Identification, and Incubation.
C)Inoculation, Incubation, Isolation, Inspection, and Identification.
D)Inspection, Inoculation, Incubation, Isolation, and Identification.

Question 20

Media that contain complex organic substances such as blood for the growth of specific bacteria are referred to as

A)enriched media.
B)general-purpose media.
C)differential media.
D)reducing media.

Question 21

Match the description below with the correct item from this list.
a.MacConkey agar
b.Phenylethyl alcohol agar
c.Nutrient agar
d.Tryptic soy agar
e.Chocolate agar
f.Xylose-lysine-deoxycholate agar
g.Thayer-Martin agar
Blood agar plate in which blood cells have been lysed; used for growing fastidious respiratory bacteria

Question 22

A medium that grows several different organisms that show visible differences in colony morphology is a __________ medium.

Question 23

Match the description below with the correct item from this list.
a.MacConkey agar
b.Phenylethyl alcohol agar
c.Nutrient agar
d.Tryptic soy agar
e.Chocolate agar
f.Xylose-lysine-deoxycholate agar
g.Thayer-Martin agar
Selective medium for gram-positive bacteria while inhibiting gram-negative bacteria

Question 24

Bacteria that appear red after gram staining are said to be gram-__________.

Question 25

Bacteria that show variation in cellular size and shape as a result of environmental conditions or age of the organisms are referred to as __________ bacteria.

Question 26

Match the description below with the correct item from this list.
a.MacConkey agar
b.Phenylethyl alcohol agar
c.Nutrient agar
d.Tryptic soy agar
e.Chocolate agar
f.Xylose-lysine-deoxycholate agar
g.Thayer-Martin agar
General-purpose medium frequently used as the base medium of other agar plate types

Question 27

Match the description below with the correct item from this list.
a.MacConkey agar
b.Phenylethyl alcohol agar
c.Nutrient agar
d.Tryptic soy agar
e.Chocolate agar
f.Xylose-lysine-deoxycholate agar
g.Thayer-Martin agar
Used for culture of stool samples to detect Salmonella

Question 28

Substances that kill bacteria are referred to as __________.

Question 29

Match the description below with the correct item from this list.
a.MacConkey agar
b.Phenylethyl alcohol agar
c.Nutrient agar
d.Tryptic soy agar
e.Chocolate agar
f.Xylose-lysine-deoxycholate agar
g.Thayer-Martin agar
Most commonly used general medium in the laboratory

Question 30

A procedure performed under sterile conditions uses the __________ technique.

Question 31

A culture that contains a single species of an organism is a __________ culture.

Question 32

A disinfectant is designed to be used only on __________ surfaces.

Question 33

A saucer-shaped liquefaction of gelatin stabs is said to have a __________ appearance.

Question 34

A common laboratory medium is nutrient broth,which contains beef extract and __________.