Deck 8: Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization

Question 1

Complementary nucleic acid hybridization is coupled with nucleic acid replication and applied repeatedly through numerous cycles.

A)Hybridization
B)Sequencing
C)Amplification (PCR)
D)Genotypic

Answer 1

C
In PCR-based methods, the target nucleic acid is amplified by nucleic acid replication that are applied repeatedly through numerous cycles.

Question 2

The target in the hybridization assay can be described as

A)originating from the unknown organism to be detected or identified.
B)a "reporter" molecule that chemically forms a complex with the single-stranded probe DNA.
C)originating from an organism of known identity.
D)All of the above are correct.

Answer 2

A
The target in the hybridization assay is the strand of DNA from the unknown organism to be detected or identified.

Question 3

Determination of the exact nucleotide sequence of a gene or gene fragment obtained from an unknown organism.

A)Hybridization
B)Sequencing
C)Amplification (PCR)
D)Genotypic

Answer 3

B
The nucleotide sequence of a microorganism's genome is the blueprint for that organism, so knowledge of its nature is a valuable tool in identifying infectious agents in clinical specimens.

Question 4

Two nucleic acid strands (a probe and a target) that have complementary base sequences specifically bond with each other and form a double-stranded molecule.

A)Hybridization
B)Sequencing
C)Amplification (PCR)
D)Genotypic

Question 5

Nucleic acid sequencing methods are based on

A)the ability of two nucleic acid strands that have complementary base sequences to specifically bond with each other and form a double-stranded molecule.
B)detecting phenotypic traits not detectable by conventional strategies.
C)the principles of complementary nucleic acid hybridization with those of nucleic acid replication.
D)determining the exact nucleotide sequence of a gene or gene fragment obtained from an organism.

Question 6

Advantages of the amplification methods are

A)ability to detect nonviable organisms.
B)ability to detect organisms that are difficult to cultivate.
C)ability to detect more quickly those organisms that grow slowly.
D)ability to quantitate infectious agent burden directly in specimens.
E)All of the above are correct.

Question 7

Target nucleic acid amplification (PCR) methods are based on

A)the ability of two nucleic acid strands that have complementary base sequences to specifically bond with each other and form a double-stranded molecule.
B)detecting phenotypic traits not detectable by conventional strategies.
C)the principles of complementary nucleic acid hybridization with those of nucleic acid replication that are applied repeatedly through numerous cycles.
D)determining the exact nucleotide sequence of a gene or gene fragment obtained from an organism.

Question 8

The probe in the hybridization assay can be described as

A)originating from the unknown organism to be detected or identified.
B)a "reporter" molecule that chemically forms a complex with the single-stranded probe DNA.
C)originating from an organism of known identity.
D)All of the above are correct.

Question 9

Hybridization methods are based on

A)the ability of two nucleic acid strands that have complementary base sequences to specifically bond with each other and form a double-stranded molecule.
B)detecting phenotypic traits not detectable by conventional strategies.
C)the principles of complementary nucleic acid hybridization with those of nucleic acid replication that are applied repeatedly through numerous cycles.
D)determining the exact nucleotide sequence of a gene or gene fragment obtained from an organism.