Deck 9: Immunochemical Methods Used for Organism Detection

Question 1

Polyclonal antibodies are those that

A)react with many different types of antigens.
B)react with many species of bacteria within the same genus.
C)react with many different antigens of one specific organism.
D)can be produced from many different species of host organisms.

Answer 1

C
Polyclonal antibodies are produced by immunizing various animals with an infectious agent and then isolating and purifying the antibodies from the animal's serum.

Question 2

The major advantage of immunofluorescent microscopy assays is that

A)extensive training is not necessary.
B)it serves as an excellent way to preserve the reaction for referral and archiving.
C)they allow visual assessment of the adequacy of a specimen.
D)fluorescent microscopes are easily accessible to most laboratories.

Answer 2

C
Because the viewing microscopist can see the cells of the specimen, a major advantage of immunofluorescent microscopy assays is that assessment of the adequacy of a specimen is possible.

Question 3

The precipitin test that involves antigen and antibody diffusing toward each other, producing a visible precipitin band at the point at which they meet is referred to as

A)double immunodiffusion.
B)counterimmunoelectrophoresis.
C)particle agglutination.
D)enzyme immunoassay.

Answer 3

A
Double immunodiffusion is a test that involves antigen and antibody diffusing toward each other to produce a precipitin band of precipitin at the meeting point.

Question 4

Monoclonal antibodies are those that

A)react with many different types of antigens.
B)react with many species of bacteria within the same genus.
C)react with many different antigens of one specific organism.
D)can be produced from many different species of host organisms.

Question 5

A test procedure that applies a specific fluorescent dye-antibody complex to a slide to which the patient specimen has been applied is referred to as

A)a solid-phase enzyme immunoassay.
B)a direct fluorescent antibody test.
C)an indirect fluorescent antibody test.
D)a radioimmunoassay.

Question 6

A modification of the Ouchterlony method that speeds up migration of an antigen and antibody by applying an electrical current is referred to as

A)double immunodiffusion.
B)counterimmunoelectrophoresis.
C)particle agglutination.
D)enzyme immunoassay.

Question 7

The immunochemical test that involves antigen present in a specimen binding to the combining sites of the antibody exposed on the surfaces of latex beads is referred to as

A)double immunodiffusion.
B)counterimmunoelectrophoresis.
C)particle agglutination.
D)enzyme immunoassay.

Question 8

The difference between latex agglutination and coagglutination is

A)the type of particle used to carry the antibody.
B)the type of target organism involved.
C)that coagglutination is less specific but more sensitive.
D)coagglutination can be used for direct antigen detection but not latex agglutination.