Deck 25: Mycoplasma and Ureaplasma

Question 1

Which antibiotic is added to mycoplasma isolation and transport media to minimize bacterial contamination?

A) Tetracycline
B) Penicillin
C) Erythromycin
D) Gentamicin

Answer 1

B
Because they lack a cell wall,the mycoplasmas are inherently resistant to the b-lactams: penicillins and cephalosporins.Penicillin is therefore added to transport and culture media to minimize bacterial contamination.

Question 2

One of the factors that allows Mycoplasma to infect the respiratory tract and the urogenital tract is that:

A) The body has a hard time detecting their presence because they have no cell wall.
B) They are virulent organisms.
C) They adhere to the epithelium of mucosal surfaces and are not eliminated by mucus secretions.
D) All of the above.

Answer 2

C
The mycoplasmas adhere to the epithelium of mucosal surfaces in the respiratory and urogenital tracts and are not eliminated by mucous secretions or urine flow.

Question 3

What disease causes the condition primary atypical pneumonia?

A) Mycoplasma hominis
B) M. urealyticum
C) M. fermentans
D) M. pneumoniae

Answer 3

D
M.pneumoniae may cause bronchitis,pharyngitis,or a relatively common respiratory infection known as primary atypical pneumonia or walking pneumonia.

Question 4

Which factor is added to media to provide cholesterol for mycoplasma-like organisms?

A) Trypticase soy digest
B) 20% fetal calf serum
C) X and V factor supplement
D) 5% sheep blood

Question 5

What time frame is suitable for blood draws to diagnose Mycoplasma infections?

A) Onset of symptoms and 2 to 3 weeks later
B) Week after symptoms start and 2 months later
C) Weeks after symptoms start and 3 months later
D) Onset of symptoms and 2 months later

Question 6

What test method is used to detect antibodies to Mycoplasma spp.?

A) Complement fixation (CF)
B) Enzyme immunoassay (EIA)
C) Direct fluorescent antibody (DFA)
D) Cellulose ion exchange (CIE)

Question 7

At-risk groups for contracting walking pneumonia include all the following EXCEPT:

A) Prisoners
B) College students
C) Military personnel
D) Very young children

Question 8

What organism has been recovered in normal,sexually active females but is associated with chorioamnionitis,congenital pneumonia,and development of chronic lung disease in premature infants?

A) Mycoplasma hominis
B) Ureaplasma ureolyticus
C) M. penetrans
D) M. fermentans

Question 9

Which of the following methods is used to determine the presence of growth in mycoplasma broth media?

A) Broth turbidity
B) Presence of biofilm on surface
C) Change in phenol red pH indicator
D) Release of radiolabeled CO₂

Question 10

When Mycoplasma hominis invades the upper genitourinary tract,it can cause the following?

A) Salpingitis
B) Pyelonephritis
C) Pelvic inflammatory disease
D) All of the above

Question 11

Which of the following stains is used to enhance the visibility of mycoplasma-like colonies?

A) Gram stain
B) Acridine orange
C) Dienes stain
D) Wright stain

Question 12

Mycoplasmas are a different type of bacteria because they do not have a _____.

A) Cell wall
B) Cell membrane
C) Nuclear membrane
D) Messenger transporter

Question 13

If a newborn has symptoms of meningitis but the cerebrospinal fluid (CSF)is negative for both Gram stain and a routine culture,what organisms should be suspected?

A) Neisseria gonorrhea and Chlamydia trachomatis
B) Treponema pallidum and Borrelia burgdorferi
C) Mycoplasma hominis and Ureaplasma urealyticum
D) M. pneumoniae and C. trachomatis

Question 14

When transporting specimens for Mycoplasma culture to the laboratory,extreme care must be taken so that the specimen does not:

A) Become contaminated
B) Leak from the specimen container
C) Become overgrown with bacterial normal florae
D) Dry out

Question 15

Which of the following organisms was once called "T-strain mycoplasma"?

A) Ureaplasma spp.
B) Mycoplasma hominis
C) Mycoplasma fermentans
D) Mycoplasma orale

Question 16

Mycoplasma organisms will grow in which of the following atmospheres?

A) Ambient
B) Capnophilic
C) Anaerobic
D) All of the above

Question 17

What two organisms are associated with urogenital tract infections but have also been isolated from asymptomatic individuals?

A) Mycoplasma hominis and Ureaplasma urealyticum
B) M. hominis and M. fermentans
C) U. urealyticum and M. pneumoniae
D) M. penetrans and M. pneumoniae

Question 18

Mycoplasma sp.from patient samples are visible by which staining technique?

A) Gram stain
B) Methylene Blue
C) Acridine orange
D) All of the above

Question 19

All the following antibiotics are used to treat mycoplasma infections EXCEPT:

A) Macrolides
B) Fluoroquinolones
C) Tetracyclines
D) Penicillin

Question 20

Cultures for Mycoplasma pneumoniae should be incubated for _____.

A) 2 to 3 days
B) 5 to 7 days
C) 10 to 14 days
D) 21 days or more