Question 1

If a microscope is ________,then the user will not have to make adjustments to focus the specimen when switching between objective lenses.&#10;A) compound&#10;B) binocular&#10;C) parfocal&#10;D) monocular

Accepted Answer

Parfocal means that when switching between objective lenses, the specimen remains in focus and no adjustment is necessary.

Question 2

As the resolving power of the objective lens increases,the working distance ________.&#10;A) increases&#10;B) decreases&#10;C) increases by a factor of 10&#10;D) remains the same

Accepted Answer

The working distance decreases as the resolving power of the objective lens increases. This is because higher resolving power requires a closer distance between the lens and the specimen to achieve sharp focus.

Question 3

The ________ controls the brightness of the light emitted from the substage light source.&#10;A) iris diaphragm&#10;B) stage&#10;C) mechanical stage&#10;D) condenser lens

Accepted Answer

The iris diaphragm is responsible for controlling the brightness of the light emitted from the substage light source. It allows the user to adjust the diameter of the opening, thereby regulating the amount of light that passes through to the specimen. The stage is used to hold and move the specimen, the mechanical stage is an advanced version of the stage with more precise movement capabilities, and the condenser lens is used to focus the light onto the specimen. None of these components control the brightness of the substage light source.

Question 4

The ability to distinguish close objects as separate and distinct is known as ________.&#10;A) working distance&#10;B) field of view&#10;C) resolving power&#10;D) depth of field

Accepted Answer

Resolving power is the ability of an optical instrument to distinguish closely spaced objects as separate and distinct. Working distance refers to the distance between the object being observed and the objective lens of the microscope. Field of view is the area visible through the microscope. Depth of field refers to the range of distances in which an object can be in focus.

Question 5

The ________ is a revolving structure that holds the objective lenses.&#10;A) coarse adjustment knob&#10;B) nosepiece&#10;C) mechanical stage control knobs&#10;D) fine adjustment knob

Accepted Answer

The nosepiece is the structure that holds the objective lenses and allows for easy switching between them. The coarse adjustment knob, mechanical stage control knobs, and fine adjustment knob are all part of the focusing mechanism of the microscope and do not hold the objective lenses.

Question 6

As the user switches to a higher-power lens to increase total magnification,the field of view ________.&#10;A) decreases&#10;B) decreases 4&#215;&#10;C) increases&#10;D) remains the same

Accepted Answer

As the total magnification increases with a higher-power lens, the field of view will decrease. This is because higher magnification means a smaller area of the specimen is visible.

Question 7

At a total magnification of 100&#215;,the diameter of the field of view is 6 mm.If the total magnification is increased to 1000&#215;,the diameter of the field of view is ________.&#10;A) 0.6 mm&#10;B) 0.06 mm&#10;C) 60 mm&#10;D) 600 mm

Accepted Answer

The diameter of the field of view decreases inversely with the increase in magnification. If the magnification increases by a factor of 10 (from 100× to 1000×), the diameter of the field of view decreases by the same factor, from 6 mm to 0.6 mm.

Question 8

The proper way to carry a microscope is in front of your body with one hand gripping the ________ and the other hand supporting the ________.&#10;A) arm; base&#10;B) head; stage&#10;C) objective lenses; base&#10;D) ocular lenses; arm

Accepted Answer

The arm of the microscope provides stability and balance, while the base supports the weight of the microscope. Gripping the arm with one hand and supporting the base with the other hand is the proper way to carry a microscope to avoid damaging any delicate components. Choice B, head and stage, are incorrect because the head of the microscope should not be used for carrying since it is not as stable as the arm. Choice C, objective lenses and base, and Choice D, ocular lenses and arm, are both incorrect because the lenses are delicate and should not be touched while carrying the microscope.

Question 9

The coarse and fine adjustment knobs are used to ________.&#10;A) nosepiece&#10;B) make initial and subsequent focusing adjustments when viewing a specimen.&#10;C) secure the specimen on the stage&#10;D) illuminate the specimen

Accepted Answer

The coarse and fine adjustment knobs are used to make initial and subsequent focusing adjustments when viewing a specimen. The coarse adjustment knob is used for rough focusing, while the fine adjustment knob is used for precise focusing.

Question 10

All microscope lenses should be cleaned with ________.&#10;A) lens paper&#10;B) tissue paper&#10;C) paper&#10;D) paper towels

Accepted Answer

Lens paper is specifically designed to clean delicate surfaces like microscope lenses without leaving behind debris or scratches. Tissue paper, paper, and paper towels can all leave behind fibers or scratches that could damage the delicate lenses.

Question 11

If a student uses a parfocal compound light microscope,which of the following would be true?&#10;A) The focus will have to be adjusted using the mechanical stage control knob.&#10;B) After the initial focus adjustments are made, the image should remain in focus as the specimen is viewed with each objective lens.&#10;C) The focus will have to be adjusted using the coarse adjustment knob.&#10;D) The focus will have to be adjusted using the fine adjustment knob.

Accepted Answer

The answer of If a student uses a parfocal compound...

Question 12

If the microscope is not parfocal,the user should make focusing adjustments using the ________ adjustment knob when viewing a specimen under high power.&#10;A) coarse&#10;B) condenser&#10;C) stage&#10;D) fine

Accepted Answer

The answer of If the microscope is not parfocal,the user...

Question 13

The total magnification of a specimen can be calculated by multiplying the ocular lens magnification by the ________ lens magnification.&#10;A) low power&#10;B) scanning&#10;C) high power&#10;D) objective

Accepted Answer

The answer of The total magnification of a specimen can...

Question 14

Assume a structure within a specimen fills approximately 25 percent of the diameter of the field of view.If the field diameter is known to be 3.5 mm,calculate the size of the structure.&#10;A) 0.14 mm&#10;B) 14 mm&#10;C) 0.875 mm&#10;D) 0.0875 mm

Accepted Answer

The answer of Assume a structure within a specimen fills...

Question 15

As the coarse adjustment knob is turned,the stage (or in some cases the nosepiece)________.&#10;A) remains stationary&#10;B) moves forward or backward&#10;C) moves up or down, depending on the direction that the knob is turned&#10;D) moves left or right, depending on the direction that the knob is turned

Accepted Answer

The answer of As the coarse adjustment knob is turned,the...

Question 16

Calculate the total magnification of a specimen if the magnification of the ocular lens is 10&#215; and the magnification of the objective lens is 45&#215;.&#10;A) 450&#215;&#10;B) 4.5&#215;&#10;C) 45&#215;&#10;D) 4500&#215;

Accepted Answer

The answer of Calculate the total magnification of a specimen...

Question 17

The distance between the objective lens and the microscope stage is called the ________.&#10;A) mechanical stage&#10;B) working distance&#10;C) resolving power&#10;D) total magnification

Accepted Answer

The answer of The distance between the objective lens and...

Question 18

To avoid damaging a lens or breaking a slide,the user should always begin viewing a specimen with the ________ power objective lens.&#10;A) medium&#10;B) lowest&#10;C) oil immersion&#10;D) highest

Accepted Answer

The answer of To avoid damaging a lens or breaking...

Question 19

Inversion of image means that when viewing a specimen with a light microscope,the image that you see will be ________ and ________.&#10;A) distorted; magnified&#10;B) magnified; inverted&#10;C) inverted; reversed&#10;D) magnified; reversed

Accepted Answer

The answer of Inversion of image means that when viewing...

Question 20

The ________ lens is known as the oil immersion lens.&#10;A) 4&#215;&#10;B) 10&#215;&#10;C) 40&#215;&#10;D) 100&#215;

Accepted Answer

The answer of The ________ lens is known as the...

Deck 2: Care and Use of the Compound Light Microscope