Deck 22: Genomics I: Analysis of DNA

Question 1

A _______ is a combination of a plasmid vector and phage $\lambda$ .

A)contig
B)artificial chromosome
C)cosmid
D)positional clone
E)transposable element

Answer 1

cosmid

Question 2

The process of in situ hybridization is used for which of the following ?

A)Cytogenetic mapping
B)Physical mapping
C)Linkage mapping

Answer 2

A

Question 3

BAC stands for bacterial artificial chromosome.

Answer 3

True

Question 4

A site that has variation within the members of the population is said to be ________.

A)Monomorphic
B)Polymorphic
C)Trimorphic

Question 5

Which of the following acronyms describes a technique used in chromosome painting?

A)RFLP
B)VNTR
C)STR
D)FISH
E)STS

Question 6

An RFLP marker may be used to predict the likelihood that an individual carries a trait for a specific disease.

Question 7

If all members of a population have the identical DNA sequence at a given site, that site is said to be _______.

A)Monomorphic
B)Polymorphic
C)Trimorphic

Question 8

What does physical mapping provide that the other types of genetic mapping do not?

A)Recombination distances between genes
B)Number of nucleotide base-pairs between genes
C)Relationship of the gene to specific chromosomal banding patterns

Question 9

Metagenomics refers to the molecular analysis of the entire genome of a species.

Question 10

The most common microsatellite in humans is ____.

A)(AT)₁₀₀
B)(CA)_n
C)(TG)_n
D)(CAT)_n

Question 11

Map units correspond to fixed physical distances.

Question 12

In a FISH procedure, the probe being used is labeled with a fluorescent tag.

Question 13

________ uses the unique banding patterns of a chromosome to determine the general location of a gene.

A)Physical mapping
B)Cytogenetic mapping
C)Linkage mapping

Question 14

The human genome project's first draft took approximately _______ years.

A) 5
B) 13
C) 20
D) 50
E) 2

Question 15

A series of clones that contain overlapping pieces of a chromosome are called a _______.

A) contig
B) artificial chromosome
C) cosmid
D) positional clone
E) transposable element

Question 16

The term that best describes how the components of a genome interact to produce an organism's traits is:

A)Proteomics
B)Functional genomics
C)Structural genomics
D)Genomics

Question 17

_________ involves an examination of how the proteins encoded by genes interact to produce cell and tissue types.

A)Proteomics
B)Functional genomics
C)Structural genomics
D)Genomics

Question 18

YAC stands for yeast artificial cosmid.

Question 19

The human genome consists of approximately _______ base pairs of DNA.

A) 100,000
B) 1 million
C) 1 billion
D) 3 billion
E) 2 trillion

Question 20

Which of the following procedures best determines the relative order, but not precise location, of a series of genes on a chromosome?

A)Physical mapping
B)Cytogenetic mapping
C)Linkage mapping

Question 21

Shotgun sequencing is precise enough to achieve a complete genomic sequence without the need for other mapping methods.

Question 22

In shotgun sequencing, how is a complete sequence of a genome reconstructed?

A)The entire genome is directly sequenced chromosome by chromosome.
B)The genome is fragmented and sequences of each fragment are arranged so that overlapping regions are identified.
C)The sequences of a large number of individuals is obtained and "averaged" to obtain the complete sequence.
D)The position of each marker and gene are first mapped relative to regions for which the genomic sequence is not known.

Question 23

To map a gene by chromosome walking, the sequence of the gene to be mapped is required.

Question 24

Which of the following samples would NOT be suitable for a metagenomics-based approach?

A)Water samples from different bodies of water with different temperatures, salinities, and acidities
B)A soil sample from a farm where a new planting schedule has been implemented
C)A water sample from an industrial site that has been contaminated with pollutants
D)A fecal sample from a patient with irritable bowel syndrome
E) A homogenous population of skin cancer cells collected from a patient biopsy

Question 25

Which of the following is the most direct benefit of high-throughput Sanger sequencing?

A)Bases can be identified as soon as they are added to the template.
B)RNA can be directly sequenced without first reverse transcribing it into DNA.
C)An entire chromosome can be sequenced without disrupting it into smaller fragments.
D)Many DNA samples can be sequenced simultaneously.

Question 26

The method of sequencing genomic fragments cloned straight from the genome is:

A)Cytogenetic mapping
B)Shotgun sequencing
C)Southern blotting
D)Polymerase chain reaction

Question 27

Comparative genomics attempts to define evolutionary relationships.

Question 28

Metagenomics is a common approach for studying the genes of a clonal population of bacteria in the laboratory.

Question 29

An amplified restriction fragment length polymorphism is a combination of the procedures for producing RFLP and STS markers.

Question 30

In positional cloning, which method is used to identify clones that are progressively closer to a gene of interest?

A)Chromosome painting
B)Cytogenetic mapping
C)Microsatellite analysis
D)Chromosome walking

Question 31

In sequencing by synthesis (SBS) methods, the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

Question 32

Cytogenetic mapping is accurate to within 500,000 base pairs, but not over this value.