Deck 19: Recombination and Transposition at the Molecular Level

Question 1

Which enzyme is unique to the retro elements?

A)Reverse transcriptase
B)Reverse resolvase
C)Transposase
D)Integrase
E)Reverse integrase

Answer 1

A

Question 2

Which of the following genes was mobile in McClintock's experiments with mutable sites in corn?

A)Ds
B)C
C)Sh
D)Wx
E)All of the answers are correct

Answer 2

A

Question 3

Which of the following describes attP and attB of bacteriophage $\lambda$ ?

A)Specific proteins in the cell membrane
B)A unique set of phospholipids that identify the organelle of interest
C)Glycoproteins in the cell wall
D)Specific DNA sequences that are recognized by integrase

Answer 3

Specific DNA sequences that are recognized by integrase

Question 4

Bacteriophage $\lambda$ inserts into a bacterial chromosome using which of the following?

A)Nucleotide excision repair
B)Endonuclease activity
C)Site-specific recombination
D)DNA gap repair

Question 5

Which of the following is technically not part of the genetic information of a transposable element?

A)Long-terminal repeats
B)Inverted repeats
C)Direct repeats
D)Transposase

Question 6

In

A)RecG
B)RecA
C)RecBCD
D)RuvABC
E)coli, a mutation in the genes encoding which of the following proteins would disable the ability of the cell to recognize double-stranded breaks and conduct recombination during meiosis?

Question 7

The formation of a D-loop is associated with which of the following models of recombination?

A)Double-stranded break model
B)Holliday model
C)Single-stranded break model

Question 8

The individual who first proposed the existence of transposable elements was ____.

A)Sturtevant
B)Morgan
C)McClintock
D)Franklin
E)Watson

Question 9

Which of the following best describes the function of the RecA protein in

A)Promotes strand invasion and formation of the D loop
B)Recognizes double-stranded breaks in the DNA
C)Is involved in the initial formation of the Holliday junctions
D)Resolves Holliday junctions into separate chromosomes
E)coli recombination?

Question 10

Long-terminal repeats are unique to which group of transposons?

A)Composite transposon
B)Non-viral retro element
C)Viral-like retro element
D)Replicative transposon
E)Insertion sequence

Question 11

Which of the following may account for the process of gene conversion?

A)DNA mismatch repair
B)DNA gap repair
C)Photolyase activity
D)Nucleotide excision repair

Question 12

Which of the following processes accounts for the tremendous variation in antibody structure?

A)RNA editing
B)Site-specific recombination
C)Integrase activity
D)Physiological adaptation
E)Hot spots of mutation in the immunoglobin genes

Question 13

Which of the following domain types is unique to the heavy chain of the polypeptide?

A)V
B)D
C)J
D)C

Question 14

The Holliday model is used to explain which of the following?

A)Recombination between homologous chromosomes
B)Recombination between sister chromatids
C)The migration of transposons
D)The replication of transposons
E) The generation of antibody diversity

Question 15

Insertion sequences can be identified by which of the following?

A)Presence of an integrase enzyme
B)High level of mutation
C)Presence of inverted repeats at the end of the sequence
D)Presence of a reverse transcriptase enzyme

Question 16

Recombination between sister chromatids produces new combinations of alleles that may be beneficial to the species.

Question 17

Which of the following most likely accounts for the majority of the recombination events during both DNA repair and meiosis?

A)A nick in only one strand of the DNA
B)Double-stranded breaks in DNA
C)DNA gap repair synthesis
D)Heteroduplex formation

Question 18

Transposons that utilize an RNA intermediate for transposition are called _____.

A)IS elements
B)Retroviruses
C)Retrotransposons
D)Non-transposable elements

Question 19

RAG1 and RAG2 proteins are associated with which of the following?

A)Recombination during prophase I of meiosis
B)Nucleotide excision repair
C)Transposition
D)Integration of a bacteriophage into the host genome
E)Site-specific recombination in antibody genes

Question 20

Which of the following enzymes is required for a transposon to move?

A)Integrase
B)Jumpase
C)Splicase
D)Transposase

Question 21

Which of the following is an application of transposon tagging?

A)To test if a transposon is replicative
B)As a tool to clone novel genes
C)To track the movement of transposons between species
D)To inactivate transposons in a genome

Question 22

Transposable elements in bacteria are commonly called insertion sequences.

Question 23

In eukaryotic organisms, a single-stranded break in the DNA is required before recombination can begin during meiosis.

Question 24

A bacteriophage that has integrated into the bacterial chromosome is called a prophage.

Question 25

Ty is an example of a retro element in the human genome.

Question 26

To date, there are not any indications that transposable elements offer a selective advantage to a species.

Question 27

The process of recombination occurs during metaphase I of meiosis.

Question 28

High numbers of LINEs and SINEs may be due to transposable element activity.

Question 29

Which of the following is an example of a transposable element in humans?

A)Alu
B)P elements
C)Mu
D)Ty

Question 30

Transposase is involved with removing the transposable element from its current location.

Question 31

A heteroduplex is a DNA double helix that contains mismatches.

Question 32

The integrase enzyme is responsible for inserting bacteriophage $\lambda$ into the host genome.

Question 33

The human genome contains a unique gene for each immunoglobulin produced.

Question 34

What is the selfish DNA theory?

A)A theory that explains how DNA point mutations are repaired within the genome.
B)A theory that explains how transposable elements are repaired within the genome.
C)A theory that attempts to explain the proliferation of transposable elements in a genome by comparing it to the action of a parasite.
D)A theory that attempts to explain how the proliferation of transposons improves the evolutionary advantage of the host organism.

Question 35

Site-specific recombination occurs between homologous sequences of DNA.