Deck 8: Basic Serologic Laboratory Techniques

Question 1

A dilution is a(n):

A) ratio of volume or number of parts of the substance to be diluted in the total volume,or parts,of the final solution.
B) indication of relative concentration.
C) frequently used measure in serologic testing.
D) all of the above.

Answer 1

indication of relative concentration.

Question 2

A central concept of serologic testing is:

A) antigen-antibody interaction.
B) determination of antibody composition.
C) determination of antigen titer.
D) rise in antibody titer.

Answer 2

determination of antigen titer.

Question 3

Automatic pipettes have the advantage of:

A) being fast.
B) allowing repetitive measurement of solutions.
C) delivering equal volumes of solutions.
D) all of the above.

Answer 3

all of the above.

Question 4

Match the term with the appropriate description.
Graduated pipette

A)Delivers the amount of liquid contained between two calibration marks on the pipette.
B)The letters TD (to deliver)appear on the pipette.

Question 5

MATCHING
Match the term with the appropriate definition.

Chyle

A)Rupturing of red blood cells that produces a pink or reddish color to serum or plasma
B)Emulsion of fat globules that often appears in serum after eating,during digestion
C)Yellowish color to plasma or serum

Question 6

Complement can be inactivated in human serum by heating to _____° C.

A) 25
B) 37
C) 45
D) 56

Question 7

MATCHING
Match the term with the appropriate definition.

Hemolysis

A)Rupturing of red blood cells that produces a pink or reddish color to serum or plasma
B)Emulsion of fat globules that often appears in serum after eating,during digestion
C)Yellowish color to plasma or serum

Question 8

A meniscus is the:

A) curvature in the top surface of a liquid.
B) zero mark on a pipette.
C) last marking on a serologic pipette.
D) flat line of liquid in a pipette.

Question 9

A graduated pipette can be used when:

A) extreme accuracy is needed.
B) very precise accuracy is needed.
C) great accuracy is not needed.
D) speed is more important than precision.

Question 10

Complete the final dilution row (1,2,3)in the table below using the following dilutions:

A)1:3
B)1:4
C)1:64
D)1:256

Question 11

Match the term with the appropriate description.
Serologic pipette

A)Delivers the amount of liquid contained between two calibration marks on the pipette.
B)The letters TD (to deliver)appear on the pipette.

Question 12

MATCHING
Match the term with the appropriate definition.

Icteric

A)Rupturing of red blood cells that produces a pink or reddish color to serum or plasma
B)Emulsion of fat globules that often appears in serum after eating,during digestion
C)Yellowish color to plasma or serum

Question 13

To dilute a serum specimen for a 1:10 dilution,

parts of serum would be added to a tube,and then

A)0.01
B)0.1
C)0.5
D)1.0
E)10
F)9
G)4.5
H)0.10

Question 14

To dilute a serum specimen for a 1:10 dilution,

parts of distilled water would be added to the 10-mL mark.

A)0.01
B)0.1
C)0.5
D)1.0
E)10
F)9
G)4.5
H)0.10

Question 15

Factors that can denature,coagulate,or alter protein molecules include:

A) heat.
B) bacteria.
C) diluting the serum specimen.
D) both a and b

Question 16

The term antibody titer is defined as:

A) the reciprocal of the highest dilution of the patient's serum.
B) a change in antibody strength.
C) the response to an antigen.
D) both b and c

Question 17

A specimen should be reinactivated when more than ______ hour(s)has (have)elapsed since inactivation.

A) 1
B) 2
C) 4
D) 8

Question 18

Serum for the detection of antibodies should be drawn during the:

A) acute phase of illness only.
B) acute and convalescent phases of illness.
C) convalescent phase of illness only.
D) acute and convalescent phases,as well as 6 months after an illness.