Deck 13: Automated Procedures

Question 1

Which of the following is a correct statement regarding measurements of turbidity?

A) Light is scattered at a determined angle.
B) It is unaffected by large particles precipitating out of a solution.
C) The reference value is recorded in units of relative light scatter.
D) It represents the scattered light in forward direction.

Answer 1

Light is scattered at a determined angle.

Question 2

A fluorescent polarization immunoassay can perform a(n):

A) microparticle enzyme immunoassay.
B) fluorescence polarization immunoassay.
C) ion capture technologies.
D) all of the above.

Answer 2

all of the above.

Question 3

Latex particles act as ______________________ in nephelometry.

A) antigen-coated reaction intensifiers
B) antigen-coated reaction stabilizers of precipitate
C) sensitivity enhancers
D) color reaction intensifiers

Answer 3

antigen-coated reaction intensifiers

Question 4

A blood specimen for testing for the presence of cryoglobulins should be:

A) immediately placed in warm water after collection.
B) immediately placed on ice after collection.
C) immediately refrigerated at 4° C.
D) not treated in any special way.

Question 5

A fundamental prerequisite for nephelometric protein quantitation is the:

A) dissolution of large protein molecules.
B) formation of a macromolecular complex.
C) pH of the solution.
D) ionic strength of the solution.

Question 6

The principle of flow cytometry is based on:

A) antigen-antibody reactions.
B) the formation of immune complexes.
C) the reaction of dyes with the cellular component of interest.
D) the reaction of dyes with immune complexes.

Question 7

Immunophenotyping includes the detection or identification of:

A) CD34+ stem cells.
B) minimal residual disease in patients with leukemia.
C) T and B lymphocytes.
D) all of the above.

Question 8

Nephelometry is less expensive initially than other forms of testing.

Question 9

In nephelometry measurement,if antigen overwhelms the quantity of antibody,the measured signal:

A) increases.
B) decreases.
C) remains the same.
D) fluctuates between high and low.

Question 10

In nephelometry measurement,a __________________method is used.

A) variable-time
B) fixed-time
C) fluorescent-tagging
D) competitive immunoassay

Question 11

Laser is an acronym for:

A) light amplification by stimulated emission of radiation.
B) light augmentation by stimulated emitted radiation.
C) light amplified by stimulated energy radiation.
D) large angle stimulation by emitted radiation.

Question 12

Monoclonal cryoglobulins are most often:

A) IgM.
B) IgG.
C) IgA.
D) either a or b

Question 13

Cryoglobulins are proteins that precipitate or gel when cooled to:

A) -18° C.
B) 0° C.
C) 4° C.
D) 18° C.

Question 14

A photon is a:

A) basic unit of light.
B) basic unit of all radiation.
C) component of an atom.
D) component of laser light.

Question 15

The major application(s)of flow cell technology is (are):

A) identification of cells.
B) cell sorting before further analysis.
C) diagnosis of autoimmune disease.
D) both a and b

Question 16

Cryoglobulin analysis can be useful in the diagnosis of:

A) hypothermia.
B) Raynaud's phenomenon.
C) infectious hepatitis.
D) rheumatoid arthritis.

Question 17

Current fluorescent methods can perform up to _______-color analysis.

A) two
B) four
C) eight
D) 16

Question 18

A fluorochrome is a(n):

A) dye.
B) immunologic reagent.
C) nonspecific indicator.
D) both a and b

Question 19

The most frequently used fluorochrome(s)is (are):

A) fluorescein isothiocyanate (FITC).
B) phycoerythrin (PE).
C) peridinin chlorophyll protein (PerCP).
D) all of the above.

Question 20

Nephelometry can be used to assay all the following except:

A) IgM.
B) IgG.
C) IgD.
D) IgA.

Question 21

The most common type of laser light used in laboratory instrumentation is neon.

Question 22

Microbial contamination can produce erroneous results in nephelometry.

Question 23

MATCHING
Match the activity with the appropriate phase of testing related to laboratory automation.

Documentation of quality control data

A)Preanalytical
B)Analytical
C)Postanalytical

Question 24

MATCHING
Match the activity with the appropriate phase of testing related to laboratory automation.

Specimen labeling

A)Preanalytical
B)Analytical
C)Postanalytical

Question 25

Monoclonal antibodies can produce spurious results in nephelometry.

Question 26

Laser light is concentrated.

Question 27

Lasers sort the energy in atoms and molecules,concentrate it,and release it in powerful waves.

Question 28

MATCHING
Match the activity with the appropriate phase of testing related to laboratory automation.

Validation of results

A)Preanalytical
B)Analytical
C)Postanalytical

Question 29

Laser light is multicolored.

Question 30

MATCHING
Match the activity with the appropriate phase of testing related to laboratory automation.

Quality control

A)Preanalytical
B)Analytical
C)Postanalytical