Question 1

The primary antibody in a Western blot .&#10;A)binds to the nitrocellulose membrane&#10;B)is conjugated to a detection system&#10;C)is used to block non-specific binding of the secondary antibody&#10;D)binds to the specific protein

Accepted Answer

The primary antibody is the one that binds specifically to the desired protein in the sample. It is then typically detected using a secondary antibody conjugated to a detection system, but the primary antibody itself is the one that specifically binds to the protein of interest.

Question 2

When a virus displays multiple peptide sequences,this is termed .&#10;A)phage array&#10;B)yeast two hybrid system&#10;C)phage display&#10;D)protein microarray

Accepted Answer

When a virus displays multiple peptide sequences, it is referred to as phage display. The other options (phage array, yeast two hybrid system, protein microarray) do not specifically refer to the display of multiple peptide sequences on a virus.

Question 3

Why might proteins be tagged?&#10;A)purification&#10;B)identification&#10;C)anchoring&#10;D)all of the above

Accepted Answer

Proteins can be tagged for various reasons such as purification (to isolate and purify only the desired protein), identification (to track and locate a specific protein), and anchoring (to anchor the protein in a particular location). Therefore, option D, "all of the above," is the best choice.

Question 4

What is the proteome?&#10;A)The total set of proteins in the cell at any given point.&#10;B)The number of genes that are expressed in a specific growth condition.&#10;C)The total set of proteins encoded by the organism's genome.&#10;D)The number of expressed genes.

Accepted Answer

The answer of What is the proteome?&#10;A)The total set of...

Question 5

All of the following are appropriate methods for investigating protein-protein interactions except .&#10;A)mass spectrometry&#10;B)two hybrid analysis&#10;C)electrophoretic mobility shift assays&#10;D)co-immunoprecipitation

Accepted Answer

The answer of All of the following are appropriate methods...

Question 6

A method that uses an antibody to detect a specific protein is called .&#10;A)SDS-PAGE&#10;B)Western blot&#10;C)Mass Spectrometry&#10;D)Yeast two hybrid system

Accepted Answer

The method that uses an antibody to detect a specific protein is called a Western Blot. SDS-PAGE is a method for separating proteins based on their size, Mass Spectrometry is a technique used for identifying proteins or other molecules based on their mass and Yeast Two-Hybrid system is a technique used to study protein-protein interactions. None of them involve the use of antibodies to detect specific proteins.

Question 7

Which method allows a researcher to find a gene based upon the protein that it encodes?&#10;A)Co-immunoprecipitation&#10;B)Phage display&#10;C)RT-PCR&#10;D)Intein trapping

Accepted Answer

Phage display is a technique that allows researchers to study protein interactions by displaying proteins (or peptides) on the surface of a bacteriophage. If the protein of interest is known, libraries of DNA sequences can be screened to find the gene that encodes for the interacting protein or the protein of interest itself. This method is particularly useful for identifying the gene based on the protein it encodes because it allows for the direct selection of peptides or proteins that bind to a specific target, which can then be used to identify the corresponding gene.

Question 8

Inteins .&#10;A)are protein self-splicing sequences&#10;B)are portions of mRNA that are spliced out of the primary transcript&#10;C)are never translated&#10;D)are protein sequences that are part of functional proteins

Accepted Answer

Inteins are protein self-splicing sequences that can excise themselves from within a larger protein sequence through catalytic activity. The remaining portions of the protein, called exteins, can then be joined together to form a functional protein. Inteins are not portions of mRNA and can be translated when part of a larger protein sequence.

Question 9

Proteins microarrays .&#10;A)are used to investigate protein-protein interactions&#10;B)are used to determine enzymatic activity&#10;C)help determine the binding of DNA or RNA to protein&#10;D)all of the above

Accepted Answer

Protein microarrays are used for various purposes, including investigating protein-protein interactions, determining enzymatic activity, and determining the binding of DNA or RNA to protein. Therefore, option D is the best choice as it includes all of the above-mentioned applications.

Question 10

How are proteins denatured in SDS-PAGE?&#10;A)detergent and high temperature&#10;B)high temperature only&#10;C)acylamide&#10;D)electrical charge

Accepted Answer

SDS-PAGE involves denaturing proteins with the use of a detergent (SDS) and high temperature. This causes the proteins to unfold and become negatively charged, allowing them to separate based on size when an electrical current is applied during gel electrophoresis. Acrylamide is used to create the gel matrix and does not denature the proteins on its own. Electrical charge is used to separate the denatured and negatively charged proteins based on size, but it is not the cause of denaturation.

Deck 15: Proteomics