Question 1

DNA transfer that occurs via a virus is called .&#10;A)transfection&#10;B)conjugation&#10;C)transduction&#10;D)transformation

Accepted Answer

The transfer of DNA that occurs via a virus is called transduction. Transfection refers to the introduction of foreign DNA into a eukaryotic cell. Conjugation is the transfer of DNA between bacterial cells through direct contact. Transformation is the uptake of DNA from the environment by a bacterial cell. Therefore, the best choice is C- transduction.

Question 2

The mechanism of transfer of the F-plasmid in conjugation involves .&#10;A)viruses&#10;B)donor cell death&#10;C)bidirectional replication&#10;D)rolling circle replication

Accepted Answer

The mechanism of transfer of the F-plasmid involves rolling circle replication, which enables the plasmid DNA to be transferred from a donor cell to a recipient cell through the formation of a pilus. The other options (viruses, donor cell death, and bidirectional replication) are not directly involved in the mechanism of F-plasmid transfer through conjugation.

Question 3

The uptake of naked DNA from the environment is called .&#10;A)transfection&#10;B)conjugation&#10;C)transduction&#10;D)transformation

Accepted Answer

The uptake of naked DNA from the environment is called transformation. Transfection is the introduction of DNA into eukaryotic cells using a viral vector. Conjugation is the process by which genetic material is transferred between bacterial cells. Transduction is the transfer of genetic material from one bacterium to another by a bacteriophage.

Question 4

Only the can initiate conjugation. A)F^- B)F⁺ C)Hfr D)recipient

Accepted Answer

F⁺ cells contain the F plasmid, which allows them to initiate conjugation by forming a pilus to transfer genetic material to F^- cells.

Question 5

The F-plasmid .&#10;A)is transferable&#10;B)non-transferable&#10;C)is involved in transduction&#10;D)always results in Hfr strain formation

Accepted Answer

The F-plasmid is transferable through conjugation, which is a mechanism of bacterial gene transfer. Therefore, it can be transferred from one bacterial cell to another. This transfer of genetic information is known as horizontal gene transfer.

Question 6

Hfr strains have an integrated .&#10;A)F plasmid&#10;B)R plasmid&#10;C)prophage&#10;D)lysogen

Accepted Answer

Hfr strains (High frequency of recombination strains) have an F plasmid (fertility plasmid) integrated into their chromosome, which allows them to initiate bacterial conjugation and transfer genetic material to recipient cells.

Question 7

A sex pilus forms during .&#10;A)transduction&#10;B)conjugation&#10;C)transformation&#10;D)transfection

Accepted Answer

A sex pilus is a tubular appendage that is used in bacterial conjugation, a process where genetic material is transferred from one bacterium to another. During this process, the donor bacterium produces a sex pilus that attaches to the recipient bacterium, and the two bacteria exchange genetic material through a conjugation bridge that is formed by the sex pilus. Transduction, transformation, and transfection are other mechanisms of genetic exchange in bacteria, but do not involve the formation of a sex pilus.

Question 8

In order for cells to take up naked DNA,they must be .&#10;A)dead&#10;B)actively replicating&#10;C)competent&#10;D)weak

Accepted Answer

In order for cells to take up naked DNA, they must be competent, meaning they are able to take up external DNA by natural transformation. Dead cells cannot take up DNA as the uptake is an active process that requires energy. Actively replicating cells might take up DNA, but this is not a requirement for DNA uptake. Weak cells is not a term that applies to DNA uptake.

Question 9

During conjugation,the entire double-stranded F-plasmid is transferred into the recipient.

Accepted Answer

During conjugation, only one strand of the F-plasmid is transferred to the recipient cell, where it then synthesizes a complementary strand to become double-stranded.

Question 10

A prophage .&#10;A)only occurs when a viral genome is integrated into the host genome&#10;B)is replicated along with host cell DNA&#10;C)occurs in the lysogenic life cycle of viruses.&#10;D)all of the above

Accepted Answer

A prophage is a viral genome that has integrated into the host genome and is replicated along with the host cell DNA. This integration occurs during the lysogenic life cycle of viruses. Therefore, all of the above options are correct.

Question 11

The preferential transfer of DNA fragments using viruses is called .&#10;A)generalize transduction&#10;B)specialized transduction&#10;C)conjugation&#10;D)transformation

Accepted Answer

The answer of The preferential transfer of DNA fragments using...

Deck 25: Bacterial Genetics