Deck 23: Transposition

Question 1

Which of the following has no reverse transcriptase gene associated with it?

A)HIV
B)Ty
C)copia
D)L1
E)Tn3

Answer 1

E

Question 2

Bacterial insertion sequences are the simplest of transposons and contain only short inverted repeat ends and genes for ___________.

Answer 2

transposase

Question 3

Which of the following molecules serves as a primer for reverse transcriptase during retroviral replication?

A)host snRNA
B)viral RNA
C)host tRNA
D)viral DNA
E)host DNA

Answer 3

C

Question 4

Transposable elements or _________ are DNA fragments that can move from one organism to another.

Question 5

Somatic hypermutation greatly increases antibody ______.

Question 6

_______________ is an enzyme that can synthesize DNA from an RNA template.

Question 7

Which of the following transposons does not contain LTRs or inverted repeats at its ends?

A)Ty
B)Alu elements
C)copia
D)Tn3
E)Ds

Question 8

In order for P element transfer to happen in Drosophila which of the following situations must occur?

A)a P crossed with a P female
B)a P male crossed with an M female
C)an M male crosses with a P female
D)an M male crossed with an M female
E)any male crossed with a P female

Question 9

Insertion of a Group II intron of a particular gene into an intronless version of that same gene is called_______.

Question 10

DNA sequences that resemble normal genes but do not function are called________.

Question 11

Which of the following is not necessary for retroviral insertion into the host genome?

A)RNA polymerase II
B)retroviral pol gene
C)viral protease
D)RNase H
E)reverse transcriptase

Question 12

The Ds transposable element of maize cannot transpose on its own because it lacks

A)transposase.
B)inverted repeats.
C)origin of replication.
D)antibiotic resistance.
E)Tn10.

Question 13

__________ receptor genes undergo rearrangement in a manor similar to B-cell immunoglobulin genes.

Question 14

Barbara McClintock discovered the first eukaryotic transposable element in

A)fruit flies.
B)nematodes.
C)maize.
D)fungi.
E)peas.

Question 15

Insertion sequences are transposable elements that can inactivate genes.

Question 16

Hybrid dysgenesis refers to the fact that in Drosophila a cross between a P male and an M female produce offspring that are

A)dead.
B)wingless.
C)blind.
D)sterile.
E)hairless.

Question 17

Which of the following is not true concerning immunoglobulin gene recombination signal sequences?

A)There is a conserved heptamer.
B)There is a conserved nonamer.
C)The conserved sequences are separated by a nonconserved sequence of either a 12 bp or a 23 bp sequence.
D)Recombination occurs between a 12 bp signal and a 23 bp signal.
E)Recombination only occurs between two heptamers.

Question 18

Which of the following would probably not be a characteristic of the Tn3 transposon?

A)inverted repeat sequences
B)antibiotic resistance gene(s)
C)resolvase gene
D)transposase gene
E)ori t site

Question 19

Individuals with SCID,severe combined immunodeficiency disease,have defective ________ genes,which cause loss of B-cell and T-cell function.

Question 20

In ____________transposition both strands of the original DNA move together whereas in _________ transposition one copy of the DNA moves to a new site while one copy stays in the genome.

Question 21

HERVs,human endogenous retroviruses make up about 10-20% of the human genome.

Question 22

Hybrid dysgenesis may lead to the formation of new species.

Question 23

Another name for a retrovirus is a retrotransposon.

Question 24

The recombinases RAG-1 and RAG-2 are expressed in both plasma and memory B-cells.

Question 25

Antibiotic resistance in bacteria can be transferred using insertion sequences.

Question 26

There are two transposable elements,Ds and Ac,which are necessary for variegation in maize kernel color.

Question 27

5'-ACCGTAG-GATGCCA-3'is an example of an inverted repeat sequence.

Question 28

Rearrangement of mammalian cell immunoglobulin genes is responsible for the large diversity of antibodies produced by B-cells.

Question 29

A Drosophila P female contains the P transposition repressor that prevents hybrid dysgenesis from occurring.

Question 30

The loss of bases or gain of bases is common in immunoglobulin gene rearrangement.

Question 31

Non-LTR retrotransposons,such as L1,occupy more of the human genome than exons do.

Question 32

Group II introns are non-LTR retrotransposons that are found only in bacterial cells.

Question 33

The constant region of an antibody contains the antigen binding domains.