Deck 16: Rna Processing: Other Post-Transcriptional Events

Question 1

RNA interference occurs naturally in human cells during

A)RNA viral replication.
B)DNA viral replication.
C)RNA viral transcription.
D)DNA viral transcription.
E)mRNA translation.

Answer 1

A

Question 2

If you were to treat mammary gland tissue with prolactin you would expect to see an increase in casein mRNA __________________.

Answer 2

stability (or half-life

Question 3

In which of the following organisms is trans-splicing of mRNA known to occur?

A)trypanosomes
B)yeast
C)bacteria
D)mammals
E)plants

Answer 3

A

Question 4

The function of normally occurring mRNA deamination appears to be the proper

A)cleanup of the mRNA.
B)mRNA folding.
C)mRNA splicing.
D)mRNA modification.
E)protein synthesis.

Question 5

Trypanosome mRNA editing occurs in the ______________.

Question 6

Trypanosome mitochondria contain two types of circular DNA linked together into large networks.These mitochondria are called

A)maxicircles
B)kinetoplasts
C)leucoplasts
D)plastids
E)maxi-mitochondria

Question 7

Under which of the following conditions does TfR mRNA degradation take place?

A)conversion of citrate to isocitrate
B)IRE hairpin loop formation
C)aconitase apoprotein binding to IRES
D)low iron concentrations
E)high iron concentrations

Question 8

Usually formed from foreign RNAs B

Question 9

RNA editing by nucleotide deamination can occur in which of the following cell types?

A)human brain
B)HIV infected human cells
C)red blood cells
D)Drosophila
E)All of the choices have RNA editing.

Question 10

________-splicing is the process by which exons from different genes are spliced together to form the mature mRNA.

Question 11

Usually starts as part of the stem of a naturally occurring cellular RNA stem loop A

Question 12

Which of the following enzymes removes extra nucleotides from the 5' ends of pre-tRNA?

A)RNase I
B)RNase II
C)RNase III
D)RNase E
E)RNase P

Question 13

Which of the following techniques was used by Stuart and colleagues to demonstrate mRNA editing?

A)RT-PCR
B)cDNA cloning
C)Northern Blot
D)RNase footprinting
E)mutation analysis

Question 14

Most cellular RNase P enzymes have 2 subunits,one protein subunit and I RNA subunit.RNase P from which of the following has no known RNA subunit?

A)human nuclei
B)monkey mitochondria
C)spinach chloroplast
D)yeast nuclei
E)Escherichia coli

Question 15

Which of the following RNAs is involved in mRNA editing?

A)rRNA
B)tRNA
C)gRNA
D)snRNA
E)iRNA

Question 16

Which of the following processes occurs posttranscriptionally?

A)Cap addition
B)adenosine deamination
C)Poly(A)addition
D)promoter clearance
E)unwinding

Question 17

Induces degradation of the target mRNA B

Question 18

Usually base pair imperfectly with their target mRNA A

Question 19

rRNA gene transcription occurs in the ______________.

Question 20

Which of the following enzymes is not involved in RNA interference?

A)Dicer
B)Slicer
C)Argonaute
D)Argonaute2
E)RNase P

Question 21

mRNA editing has been shown to occur in the 5' to 3' direction.

Question 22

_________ RNA is responsible for RNA interference.

Question 23

Gene silencing by RNAi can target the control region of a gene by being involved in DNA methylation.

Question 24

Dicer is an RNase III-like enzyme that cleaves dsRNA into 50-55 nt fragments.

Question 25

miRNA synthesis involves the cleavage of the double stranded stem portion of a precursor RNA by the enzyme ____________.

Question 26

Post-transcriptional processing of RNA occurs only in eukaryotic cells.

Question 27

Transposition of transposons is blocked in animal germ cells by a ping-pong and mRNA destruction mechanism involving piRNAs.

Question 28

Flowering plants have two nuclear RNA RNA polymerases,Iv and V,that are not found in animals and fungi.

Question 29

MicroRNAs function to silence gene expression primarily by blocking ____________ of targeted mRNAs.

Question 30

RNA editing in mammals involves deamination of adenosine to form __________.

Question 31

E.coli rRNA is transcribed and processed in the cytoplasm.

Question 32

Post-transcriptional processing of RNA is not important for gene expression.

Question 33

Inactivation of RNase genes involved in tRNA 3'end maturation is lethal to bacterial cells.

Question 34

Cellular mRNA levels often correlate more closely with transcription rate than with transcript stability.

Question 35

Processing a pri-miRNA to a mature miRNA is a two-step process.