Deck 14: Rna Processing I: Splicing

Question 1

Alternative splicing is involved in _________ determination in fruit flys.

Answer 1

sex

Question 2

_______________ disrupting the normal splicing consensus sequence are one way to obtain abnormal splicing patterns.

Answer 2

Mutations

Question 3

Which of the following splicing factors is required for the correct 3' splice site selection?

A)SC35
B)Slu7
C)U11
D)SF2
E)SRp55

Answer 3

Slu7

Question 4

"Snurps" are composed of

A)RNA.
B)protein.
C)DNA.
D)RNA and protein.
E)DNA and RNA.

Question 5

The binding of which of the following snRNPs to spliceosome requires ATP?

A)U1
B)U2
C)U4
D)U5
E)U6

Question 6

There are a common set of ___________ Sm proteins that are specific to each snRNA.

Question 7

Joan Steitz was one of the researchers who used __________ studies to demonstrate that U6 snRNP binds to the 5'end of the intron.

Question 8

Some introns,such as Group II introns,are self splicing.These types of introns contain catalytic RNA's,also referred to as __________.

Question 9

Which of the following is the first snRNP to bind during the assembly stage of the spliceosome cycle?

A)U1
B)U2
C)U4
D)U5
E)U6

Question 10

The catalytic center of the spliceosome appears to include

A)Mg^2+.
B)U2 and U6 snRNP.
C)the branch point region of the intron.
D)Mg²⁺ and the branch point region of the intron.
E)Mg²⁺,U2,and U6 snRNP,and the branch point region of the intron.

Question 11

Which of the following is the name for the Yeast 40S particle where mRNA splicing takes place?

A)ribosome
B)nucleolus
C)spliceosome
D)hnRNA
E)R-loop

Question 12

Which of the following is not true concerning eukaryotic mRNA processing?

A)addition of a 3' poly-A tail
B)addition of a 5' cap
C)intron splicing
D)occurs in the nucleus
E)occurs in the cytoplasm

Question 13

Exon definition involves the use of the ________ subunit of RNA polymerase II.

Question 14

The process by which introns are removed from immature mRNA is called _____________.

Question 15

The expressed regions of genes are referred to as ________ while the noncoding regions are called_______.

Question 16

Which of the following snRNP is mismatched with its function?

A)U1: base pairs with 5' splice site of mRNA.
B)U2: base pairs with the conserved sequence at splicing branch point.
C)U4: base pairs with 3'splice site of mRNA.
D)U5: associates with last nucleotide in one exon and the first nucleotide in the next exon.
E)U6: base pairs with 5' end of the intron.

Question 17

The first two bases and the last two bases in the splicing signal consensus sequence are

A)GT-AG.
B)GU-AG.
C)CU-AG.
D)GU-AC.
E)GT-TG.

Question 18

Which of the following complexes found in the spliceosome cycle is mismatched with its snRNP(s)?

A)commitment complex: U6/U4
B)A complex: U1 and U2
C)B1 complex: all snRNPs
D)B2 complex: U2,U5 and U6
E)I complex: U2,U5 and U6

Question 19

You have isolated a new species of mRNA using an oligo-dT column.You then perform an R-looping experiment to determine if the gene encoding for your mRNA contains introns.Below is a representation of the electron micrograph results.Which of the following can you conclude from your experiment?

A)The gene contains no introns.
B)The gene contains one intron.
C)The gene contains two introns.
D)The gene contains three introns.
E)The results are inconclusive.

Question 20

Which of the following experiments was useful in determining the existence of introns in the adenovirus genome?

A)DNA/DNA hybridization
B)RNA/RNA hybridization
C)R-looping experiments
D)RT-PCR
E)cDNA cloning experiments

Question 21

Immature mRNA splicing can be established by either exon definition or intron definition.

Question 22

Pre-mRNA splicing occurs in the cytoplasm.

Question 23

Introns are regions found in DNA sequences and mature mRNA sequences.

Question 24

The discovery of alternate splicing helped prove the "one gene,one protein" theory.

Question 25

Alternative splicing patterns can lead to the production of different protein products from the same gene.

Question 26

Heterogeneous nuclear RNA (hnRNA)is thought to be a precursor of mRNA.

Question 27

RNA polymerase II does not transcribe through introns,instead it jumps over them.

Question 28

The spliceosome cycle is one way for a cell to regulate gene expression.

Question 29

SC35 is an RNA binding protein that is responsible for the commitment to splice the $\beta$ -globin pre-mRNA.

Question 30

Experimental evidence demonstrates that mRNA precursors are spliced via a lariat-shaped intermediate.

Question 31

Base pairing between the 5' splice site and U1 snRNA is the only interaction essential for splicing.