Which Statement Is NOT a Way Scientists Used Mitochondrial DNA

Which statement is NOT a way scientists used mitochondrial DNA to determine where humans first evolved and how they spread?

A) Scientists sampled mtDNA from individuals currently living in many different geographic regions around the world.
B) Scientists constructed an evolutionary tree based on paternal mtDNA to trace human ancestry back to a single female.
C) Scientists used the relatively regular rate of mtDNA mutations to determine how long it had been since groups diverged from one another.
D) None of these describe how scientists used mitochondrial DNA to study human evolution.
E) All of these are methods that scientists use to study human evolution.