Protein-specific antibodies can be used to detect proteins within cells conserving the cell architecture using
A) immunofluorescence.
B) Western blot.
C) immunoprecipitation.
D) ELISA.

Question

Quizplus · Accepted Answer

Immunofluorescence is the best choice for detecting proteins within cells and conserving the cell architecture as it involves labeling specific proteins with fluorescent antibodies that emit light when excited by a specific wavelength of light. This technique allows for the visualization and localization of the target protein within the cell while preserving the cell's overall morphology. Western blot and immunoprecipitation involve extracting proteins from the cells, which may alter the cell architecture, and ELISA is typically used to detect proteins in extracellular fluids, rather than within intact cells.

Protein-Specific Antibodies Can Be Used to Detect Proteins Within Cells