You Explain to the Class That ELISA Tests May Be

You explain to the class that ELISA tests may be direct or indirect. Please select the FALSE statement regarding these tests.

A) In direct ELISAs, specific antigens in the sample are "captured" by antibodies that have been attached to the inside surface of the well
B) ELISAs have advantages over other common immunoassays in that they are easy to do and often require minimal technical skills.
C) ELISA tests are 100% accurate; unlike other immunoassays, ELISAs never yield false positive results, so additional testing is not required.
D) ELISA tests are often done in microtiter plates, allowing multiple samples to be tested all at once.
E) Indirect ELISAs require a labeled secondary antibody to detect the unlabeled first (primary) antibody.