Whole Genome or Shotgun Sequencing Requires

Whole genome or shotgun sequencing requires

A) the creation of a cDNA library.
B) the creation of overlapping DNA fragments whose sequences can be aligned into large contiguous fragments (contigs) using computer algorithms.
C) large contiguous fragments (contigs) cloned into yeast artificial chromosomes (YACs) in an ordered fashion.
D) very large amounts of DNA from the organism whose genome is being sequenced.
E) the use of a gene gun to propel DNA-coated particles at high velocities into cells.