You have determined the base sequence for a gene from Felis catus (domestic cat)that you believe may be involved in feline leukemia.The nucleotide and probable amino acid sequences are similar to retroviral proteins that become embedded in the host cell membrane.You separate the membrane fraction of feline leukemia cell lysate from the cytoplasmic fraction.What technique could you use to determine if your protein of interest is present in the membrane fraction?

Question

Quizplus · Accepted Answer

Liquid chromatography followed by mass spectrometry and two-dimensional gel electrophoresis are both techniques that can be used to identify and characterize proteins in complex mixtures, such as a membrane fraction. Liquid chromatography separates proteins based on their physical and chemical properties, and mass spectrometry provides detailed information about the molecular weight and sequence of the proteins, allowing for identification. Two-dimensional gel electrophoresis separates proteins based on their isoelectric point and molecular weight, which can also help in identifying and characterizing proteins in the membrane fraction.

You Have Determined the Base Sequence for a Gene from Felis