Scenario Avian Influenza Is a Viral Infection with Few Damaging Effects

Scenario
Avian influenza is a viral infection with few damaging effects that naturally occurs in wild birds. However, avian influenza is highly contagious and can quickly kill domesticated birds. Some human influenza viruses are similar to the avian influenza. People infected with avian influenza develop typical flu symptoms (fever, cough, muscle aches) . However, symptoms may become more severe and life-threatening. If avian influenza evolves into a form that is easily transmissible from person to person, a global pandemic of widespread human death might occur.
-Which method do you think would be best to test effectiveness of a vaccine intended for human use against avian influenza?

A) Give the vaccine to a single mouse that has the virus, and assess whether influenza symptoms develop.
B) Give the vaccine to a group of mice that have the virus, and give a placebo to another group of mice that have the virus. Assess whether influenza symptoms develop in either group.
C) Give the vaccine to a single human who has the virus, and assess whether influenza symptoms develop.
D) Give the vaccine to a group of humans who have the virus, and give a placebo to another group of humans who have the virus. Assess whether influenza symptoms develop in either group.