If ethidium bromide is used as the supravital dye in a complement dependent cytotoxicity assay, then cells injured by HLA antibody will appear orange.

Question

Quizplus · Accepted Answer

Ethidium bromide is a fluorescent dye that intercalates into DNA, and when used in a complement-dependent cytotoxicity assay, cells that have been injured and have compromised membrane integrity will take up the dye. This causes them to fluoresce orange under ultraviolet light, indicating they have been damaged by the HLA antibody.

If Ethidium Bromide Is Used as the Supravital Dye in a Complement