When DNA is extracted from a sample for HLA typing, the purity of the sample is best determined by the ratio of the sample's absorbance at 260 and 280 nm.

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The purity of DNA samples is commonly assessed by measuring the absorbance ratio at 260 nm and 280 nm (A260/A280). A ratio of ~1.8 is generally considered pure for DNA, indicating minimal protein contamination.

When DNA Is Extracted from a Sample for HLA Typing