Reverse transcriptase PCR can only be done on pure preparations of messenger RNA as ribosomal RNA and transfer RNA interfere significantly.

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Reverse transcriptase PCR can be performed on total RNA, which includes messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA), as specific primers can be designed to selectively amplify the mRNA of interest.

Reverse Transcriptase PCR Can Only Be Done on Pure Preparations