How are the probes for CGH different from probes made for gene expression analysis?

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Arrays for CGH (comparative genomic hybridization) are typically made using BAC (bacterial artificial chromosome) probes, which are large pieces of DNA that span several genes. This allows for detection of copy number variations or deletions/duplications of large regions of the genome. In contrast, gene expression analysis typically uses cDNA or oligonucleotide probes that are designed to target specific genes or transcripts. miRNA (microRNA) probes are used for miRNA expression analysis, which is a different type of analysis than CGH.PCR amplicon probes may be used in targeted sequencing studies or genotyping assays, but are not commonly used for CGH or gene expression analysis.

How Are the Probes for CGH Different from Probes Made