In the center square on one side of a hemocytometer,100 platelets were found;200 were found in the center square on the other side.Select the best course of action.
A)Report the count as 150 x 109/L.
B)Verify the count by estimating the platelet number on the blood film.
C)Recount the hemocytometer on a phase microscope.
D)Repeat the procedure-the two counts are not close enough.

Question

Quizplus · Accepted Answer

The cell counts on the two opposite sides of the hemocytometers must agree within 10% of each other.Thus if the count is 100 on one side,the other side must be no higher than 100 + (0.1 x 100)= 110.Because 200 exceeds this,the counts do not agree-an error has been made.The dilutions must be repeated and then the new dilutions counted.

In the Center Square on One Side of a Hemocytometer,100