Transcription of a bacterial gene is initiated
A) with RNA polymerase in the closed-promoter complex.
B) after release of the sigma ($\sigma$) factor.
C) with RNA polymerase in the open-promoter complex.
D) Both b and c

Question

Quizplus · Accepted Answer

Transcription of a bacterial gene is initiated with RNA polymerase in the open-promoter complex, where the DNA helix is unwound and the template strand is available for base pairing with incoming ribonucleotides. The closed-promoter complex is formed when RNA polymerase binds to the promoter region of the DNA but has not yet opened the helix, while release of the sigma factor occurs after initiation and is not a part of the initiation process. Therefore, choices A and B are incorrect. Choice D is incorrect because it includes choice B, which is incorrect.

Transcription of a Bacterial Gene Is Initiated A) with RNA σ\sigmaσ

Transcription of a Bacterial Gene Is Initiated
A) with RNA $\sigma$