Mass spectrometers are used to A) transport a molecule against its concentration gradient. B) couple the energy of ATP hydrolysis to a configurational change. C) transport H⁺ across a membrane against a pH gradient. D) identify proteins by mass/ratio peptide analysis as part of proteomic procedures.

Question

Quizplus · Accepted Answer

Mass spectrometers are commonly used in proteomics procedures to identify proteins through mass-to-charge ratio peptide analysis. This involves ionizing the protein sample and measuring the resulting ion masses, which can then be used to determine the amino acid sequence of the protein. Option A is incorrect as mass spectrometers are not used for transport of molecules. Option B is incorrect as ATP hydrolysis is not involved in the functioning of mass spectrometers. Option C is incorrect as the mentioned molecule transport of H⁺ and pH gradient is not relevant to mass spectrometers.

Mass Spectrometers Are Used to