In velocity centrifugation, a suspension is layered on top of a sucrose density gradient and centrifuged until the density of the particles matches the density of the sucrose solution.

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In velocity centrifugation, particles are separated based on their size and shape as they move through a medium (like sucrose) under centrifugal force, not by matching the density of the sucrose solution. Density gradient centrifugation, not velocity centrifugation, involves particles moving until their density matches that of the sucrose solution.

In Velocity Centrifugation, a Suspension Is Layered on Top of a Sucrose