Imagine you are studying an inducible transcription factor called X. You make a cellular lysate and carry out a series of centrifugation steps at increasing speeds to localize X. You find that prior to induction, X is in the supernatant after a fourth, very high speed ultracentrifugation step; however, after induction it is found in the pellet after only one, relatively low speed ultracentrifugation step. What might this tell you about how X is regulated? What other techniques might you carry out to confirm your results?

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The Answer of Imagine you are studying an inducible transcription factor called X. You make a cellular lysate and carry out a series of centrifugation steps at increasing speeds to localize X. You find that prior to induction, X is in the supernatant after a fourth, very high speed ultracentrifugation step; however, after induction it is found in the pellet after only one, relatively low speed ultracentrifugation step. What might this tell you about how X is regulated? What other techniques might you carry out to confirm your results?

Imagine You Are Studying an Inducible Transcription Factor Called X