Exhibit 2B
Contains information on the pK's of some common buffers.
$\begin{array}{llrc}
\text { Buffer } & \mathbf{p K} 1 & \mathbf{p K 2} & \mathbf{p K} 3 \
\hline \text { Acetate } & 4.75 & & \
\text { Ammonia } & 9.25 & & \
\text { Carbonic acid } & 6.37 & 10.20 & \
\text { Citric acid } & 3.09 & 4.75 & 5.41 \
\text { Formic Acid } & 3.75 & & \
\text { Phosphoric acid } & 2.14 & 7.20 & 12.4 \
\text { Pyruvic acid } & 2.50 & & \
\text { Tris } & 8.3 & &
\end{array}$

-Refer to Exhibit 2B. The enzyme lysozyme has an optimum pH close to 5. A suitable buffer would be:

Question

Exhibit 2B
Contains information on the pK's of some common buffers.
$\begin{array}{llrc}
\text { Buffer } & \mathbf{p K} 1 & \mathbf{p K 2} & \mathbf{p K} 3 \
\hline \text { Acetate } & 4.75 & & \
\text { Ammonia } & 9.25 & & \
\text { Carbonic acid } & 6.37 & 10.20 & \
\text { Citric acid } & 3.09 & 4.75 & 5.41 \
\text { Formic Acid } & 3.75 & & \
\text { Phosphoric acid } & 2.14 & 7.20 & 12.4 \
\text { Pyruvic acid } & 2.50 & & \
\text { Tris } & 8.3 & &
\end{array}$

-Refer to Exhibit 2B. The enzyme lysozyme has an optimum pH close to 5. A suitable buffer would be:

Quizplus · Accepted Answer

Based on the given pK values, acetate has a pK of 4.75 which is closest to the optimum pH of lysozyme. This means that acetate buffer will keep the pH close to 5 and help maintain the enzymatic activity of lysozyme at its maximum level. Carbonate buffer has a higher pK (10.20) and phosphate buffer has a lower pK (2.14 for the first dissociation and 7.20 for the second dissociation) compared to the required pH range for lysozyme. Pyruvate buffer has a pK of 2.50 which is too low for the required pH. Thus, the best choice for a suitable buffer for this reaction is acetate buffer.

Exhibit 2B Contains Information on the PK's of Some Common

Exhibit 2B
Contains Information on the PK's of Some Common