Exhibit 5A
The following diagram shows the anode, cathode, and $\mathrm { pH }$ gradient of an isoelectric focusing bed:

-Refer to Exhibit 5A. A mixture of asp, asn, and arg is placed in the bed where the pH is 7, and the current is turned on. From left to right, which best represents the final positions of the individual amino acids?

Question

Exhibit 5A
The following diagram shows the anode, cathode, and $\mathrm { pH }$ gradient of an isoelectric focusing bed:

-Refer to Exhibit 5A. A mixture of asp, asn, and arg is placed in the bed where the pH is 7, and the current is turned on. From left to right, which best represents the final positions of the individual amino acids?

Quizplus · Accepted Answer

Asp is negatively charged at pH 7, so it will migrate towards the anode (positive electrode). Asn is neutral at pH 7, so it will not migrate. Arg is positively charged at pH 7, so it will migrate towards the cathode (negative electrode). Therefore, the final positions of the individual amino acids will be asp (closest to the anode), asn, and arg (closest to the cathode).

Exhibit 5A the Following Diagram Shows the Anode, Cathode, And \mathrm

Exhibit 5A
the Following Diagram Shows the Anode, Cathode, And \mathrm