If the Asp in the chymotrypsin active site was mutated to another amino acid, which of the following would be considered an invisible mutation in that it is least likely to affect the function of the enzyme?

Question

Quizplus · Accepted Answer

Aspartic acid (Asp) and Glutamic acid (Glu) are both negatively charged amino acids at physiological pH. Mutating Asp to Glu is likely to have the least effect on the enzyme's function because the overall charge and the chemical nature of the side chain remain similar, preserving the enzyme's active site environment.

If the Asp in the Chymotrypsin Active Site Was Mutated →\rightarrow→

If the Asp in the Chymotrypsin Active Site Was Mutated $\rightarrow$