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A Dark Pigmented Colony Is Isolated from the Leg Wound

Question 14

Multiple Choice

A dark pigmented colony is isolated from the leg wound of a fisherman from Florida. The gram-negative rod grows on sheep blood and MacConkey agars. The organism provides the following reactions:
TSI: K/ A Indole: no color change Lysine: no color change1 % NaCL : no growth Arginine: purpleOrnithine: no color changeDNase: pink halo around the area of organism inoculum\begin{array}{lll}\text {TSI: K/ A } &\text {Indole: no color change }&\text {Lysine: no color change}\\\text {1 \% NaCL : no growth}&\text { Arginine: purple}&\text {Ornithine: no color change}\\\text {DNase: pink halo around the area of organism inoculum}\end{array}
The microbiologist cannot read the oxidase reaction due to the dark pigment. What can he do to help achieve a valid oxidase reaction?


A) Perform a modified oxidase test
B) Grow the organism under anaerobic conditions and retest
C) The oxidase test is not necessary for the identification of this organism
D) Grow the organism on a non-nutrient agar and retest

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