You have received a lineage of "knockout mice" from a collaborator. What method would not be useful to confirm that the mice really do not express the targeted gene in all tissues of the body?

Question

Quizplus · Accepted Answer

Neomycin sensitivity assay is not useful for confirming the absence of gene expression across all tissues. It is used to select cells that have integrated a neomycin resistance gene, not to assess the expression of specific genes at the RNA or protein level, unlike the other options which directly measure gene expression.

You Have Received a Lineage of "Knockout Mice" from a Collaborator