You Wish to Incorporate Radioactive Phosphate onto the 5′-Ends of a Fragment

You wish to incorporate radioactive phosphate onto the 5′-ends of a fragment of DNA so that it can be used as a probe on a Southern blot of genomic DNA. Which of the following enzymes is required in order for you to carry out the labeling of the DNA with radioactive phosphate?

A) DNA ligase
B) DNA polymerase
C) polynucleotide kinase
D) reverse transcriptase
E) terminal transferase