Using Restriction Enzymes,a Eukaryotic Gene Is Cut,ligated into a Bacterial

Using restriction enzymes,a eukaryotic gene is cut,ligated into a bacterial plasmid,and introduced into a strain of E.coli.To better the odds of getting expression of the eukaryotic gene in this system,you need to make certain that

A) the gene has a eukaryotic promoter for transcription.
B) the plasmid has a prokaryotic promoter for transcription.
C) the gene and the plasmid were cut with different restriction enzymes.
D) the gene product is toxic to the bacterial cell.
E) mRNA is used for ligation directly into the vector.