Name and compare two molecular methods that are used to detect mutations associated with sickle-cell disease.

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The Answer is Two molecular methods that can be used to detect the mutation associated with sickle-cell disease are Southern Blot analysis and PCR.Southern Blot: Restriction endonucleases cut the chromosomal DNA of the suspected sickle patient at a specific nucleotide base to produce smaller fragments of DNA.A probe specific for the HBB gene the gene responsible for SCD) is applied and targeted to the specific sequence.If the aberrant gene is present, it will show up as a band on 2D-gel.Qualitative: present or absent.)
PCR: Similar procedure to Southern Blot analysis, but at the end, the targeted DNA sequence is amplified a billionfold.

Name and Compare Two Molecular Methods That Are Used to Detect