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Why Might Both Restriction Enzyme Digestion and Sonication Be Required

Question 39

Multiple Choice

Why might both restriction enzyme digestion and sonication be required for the success of a shotgun sequencing approach? (Select all that apply)


A) Too many restriction fragments might otherwise be generated
B) Too few restriction fragments might otherwise be generated
C) Further validation of nucleotide sequence order and arrangement may be obtained through comparison of sequence data derived from each method
D) All responses are correct
E) No responses are relevant

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