Optimal recovery and identification of anaerobic bacteria in clinical specimens occurs when:
A)the initial incubation period is at 35°C to 37°C for 48 hours.
B)plates are incubated for at least 3 days before discarding as negative.
C)the initial incubation period is at 35°C to 37°C for 24 hours.
D)thioglycollate broth can be inspected daily for 3 days.

Question

Quizplus · Accepted Answer

Inoculated plates should be immediately incubated under anaerobic conditions at 35°C to 37°C for 48 hours.In general,cultures should not be exposed to oxygen until after 48 hours' incubation;anaerobes are most sensitive to oxygen during their long phase of growth.In addition,colonial morphologic characteristics often dramatically change between 24 and 48 hours.

Optimal Recovery and Identification of Anaerobic Bacteria in Clinical Specimens