Solved

A Screening of a GadA- β\beta -Gal Fusion Protein in E β\beta

Question 6

Multiple Choice

A screening of a GadA- β\beta -gal fusion protein in E. coli is performed on medium containing X-Gal. White and blue colonies are observed after incubation. Which of the following statements is NOT true regarding blue/white selection?


A) Blue colonies result from X-Gal hydrolyzed by the fusion protein's β\beta -galactosidase moiety.
B) White colonies result from cells with a disrupted gene encoding a positive regulator of GadA.
C) Galactose released from X-Gal hydrolysis does not contribute to a colony's blue color.
D) White colonies indicate hydrolysis of X-Gal catalyzed by β\beta -galactosidase.
E) All of the above.

Correct Answer:

verifed

Verified

Unlock this answer now
Get Access to more Verified Answers free of charge

Related Questions

Unlock this Answer For Free Now!

View this answer and more for free by performing one of the following actions

qr-code

Scan the QR code to install the App and get 2 free unlocks

upload documents

Unlock quizzes for free by uploading documents