A screening of a GadA- -gal fusion protein in E. coli is performed on medium containing X-Gal. White and blue colonies are observed after incubation. Which of the following statements is NOT true regarding blue/white selection?
A) Blue colonies result from X-Gal hydrolyzed by the fusion protein's -galactosidase moiety.
B) White colonies result from cells with a disrupted gene encoding a positive regulator of GadA.
C) Galactose released from X-Gal hydrolysis does not contribute to a colony's blue color.
D) White colonies indicate hydrolysis of X-Gal catalyzed by -galactosidase.
E) All of the above.
Correct Answer:
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